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Crystal structure of a protein, structurally related to glycosyltransferases, encoded in the Rhodobacter blasticus atp operon
Örebro University, Department of Natural Sciences. (Molekylär biokemi)
Örebro University, Department of Natural Sciences. (Molekylär biokemi)ORCID iD: 0000-0003-3315-8835
2008 (English)In: Biochimica et Biophysica Acta - Proteins and Proteomics, ISSN 1570-9639, E-ISSN 1878-1454, Vol. 1784, no 2, p. 379-384Article in journal (Refereed) Published
Abstract [en]

The F1-ATP synthase atp operon in the proteobacterium Rhodobacter blasticus contains six open reading frames, encoding six hypothetical proteins. Five of these subunits, in the stoichiometry (ab)3gde make up the catalytic F1-ATP synthase complex similarly in bacteria, chloroplasts and mitochondria. The sixth gene of the Rb. blasticus atp operon, urf6, shows very little sequence homology to any protein of known structure or function. The gene has previously been cloned, the product (called majastridin) has been heterologously expressed in Escherichia coli, and purified to high homogeneity (Brosché et al. (1998) Eur. J. Biochem. 255: 87-92). We have solved the X-ray crystal structure and refined a model of majastridin to atomic resolution. Here we present the crystal structures of apo-majastridin and the complex of majastridin with Mn2+ and UDP and show it has extensive structural similarity to glycosyltransferases (EC 2.4). This is the first structure determined from a new group of distantly related bacterial proteins of at least six members. They share the identical amino acids that bind Mn2+and a triplet of amino acids in the putative sugar-binding site.

Place, publisher, year, edition, pages
Amsterdam: Elsevier/North Holland , 2008. Vol. 1784, no 2, p. 379-384
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy) Medical and Health Sciences
Research subject
Biochemistry
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URN: urn:nbn:se:oru:diva-4153DOI: 10.1016/j.bbapap.2007.11.005OAI: oai:DiVA.org:oru-4153DiVA, id: diva2:138452
Available from: 2008-10-14 Created: 2008-10-14 Last updated: 2017-12-14Bibliographically approved

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Enroth, CristoferStrid, Åke

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Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)Medical and Health Sciences

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