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Alpha-1-adrenergic inositol trisphosphate production in brown adipocytes is Na+ dependent
The Wenner-Gren Institute, University of Stockholm, Stockholm, Sweden.ORCID iD: 0000-0001-8889-5803
The Wenner-Gren Institute, University of Stockholm, Stockholm, Sweden.
1987 (English)In: Biochimica et Biophysica Acta, ISSN 0006-3002, E-ISSN 1878-2434, Vol. 930, no 3, p. 438-445Article in journal (Refereed) Published
Abstract [en]

In order to investigate the ionic requirements for inositol trisphosphate production, brown adipocytes were prelabelled with myo-[3H]inositol and the formation of inositol trisphosphates and inositol bisphosphates as a consequence of alpha 1-adrenergic stimulation was monitored. Omission of Ca2+ from the incubation medium diminished the norepinephrine-induced increase in inositol trisphosphate levels, but it would seem that this reduction can be fully accounted for by a decreased level of the 'inactive' isomer inositol 1,3,4-trisphosphate. Omission of Na+ fully abolished the norepinephrine-induced inositol trisphosphate response. However, it was observed that the presence of Li+ in the incubation medium could fully reconstitute the ability of the cells to yield the early response of inositol trisphosphate production; Li+ could, however, not substitute for Na+ in the entire alpha 1-adrenergic cellular pathway. It was concluded that the Na+-dependent step is found in the coupling mechanism between the alpha 1-receptor and the activation of the phosphodiesterase responsible for inositol trisphosphate production. Thus, all events in the alpha 1-adrenergic pathway which are consequences of IP3 production should appear to be Na+-dependent in these cells.

Place, publisher, year, edition, pages
Elsevier, 1987. Vol. 930, no 3, p. 438-445
Keywords [en]
Inositol phosphate, Sodium, Lithium, Calcium, Noradrenaline, α-Adrenergic receptor, (Hamster brown adipose tissue)
National Category
Medical and Health Sciences Cell Biology
Identifiers
URN: urn:nbn:se:oru:diva-79045DOI: 10.1016/0167-4889(87)90017-6ISI: A1987K316500017PubMedID: 2820506Scopus ID: 2-s2.0-0023430744OAI: oai:DiVA.org:oru-79045DiVA, id: diva2:1386282
Available from: 2013-10-21 Created: 2020-01-17 Last updated: 2020-01-27Bibliographically approved

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