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Immunohistochemical changes in the expression of HSP27 in exercised human vastus lateralis muscle
Örebro University, School of Health and Medical Sciences. (RISPA)
Institute of Sports Medicine, Bispebjerg Hospital and Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark.
Institute of Sports Medicine, Bispebjerg Hospital and Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark.
Institute of Sports Medicine, Bispebjerg Hospital and Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark.
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2008 (English)In: Acta Physiologica, ISSN 1748-1708, E-ISSN 1748-1716, Vol. 194, no 3, p. 215-222Article in journal (Refereed) Published
Abstract [en]

Aim: The role of HSP27 in the adaptive process of skeletal muscle to exercise, especially in humans, is not well understood. The objective of this study was to investigate immunohistochemical changes in HSP27 expression in human vastus lateralis muscle following resistance and endurance exercises.

Methods: Two different exercise protocols were used: (1) one-leg ergometer cycling (EC, n = 6) consisting of two 30-min bouts at 40% and 75% of peak oxygen uptake, respectively, and (2) leg extension resistance exercise (RE, n = 9) including 10 sets of eight repetitions at a load corresponding to 70% of one maximal repetition (1RM). Immunohistochemistry using specific monoclonal antibodies was used to determine the location of HSP27 protein in muscle biopsies from human vastus lateralis.

Results: Our results show that RE, but not EC, induced a significant appearance of scattered accumulations of HSP27 protein in muscle fibres from five of nine subjects. The number of fibres with accumulation of HSP27 in RE ranged from 0% to 32% with a mean of 6.3% of the total number of fibres.

Conclusion: We conclude that this rapid HSP27 protein relocation after RE is an important player in the cellular remodelling of human muscle fibres in response to exercise involving high-force contractions, but not in response to endurance exercises.

Place, publisher, year, edition, pages
Blackwell Publishing, 2008. Vol. 194, no 3, p. 215-222
Keywords [en]
adaptation, endurance and resistance exercise, fibre type, heat shock protein, stress response
National Category
Physiology
Research subject
Physiology
Identifiers
URN: urn:nbn:se:oru:diva-4751DOI: 10.1111/j.1748-1716.2008.01875.xISI: 000259863100005PubMedID: 18489727Scopus ID: 2-s2.0-53549112177OAI: oai:DiVA.org:oru-4751DiVA, id: diva2:139050
Funder
Swedish National Centre for Research in SportsAvailable from: 2008-11-24 Created: 2008-11-24 Last updated: 2018-09-24Bibliographically approved
In thesis
1. Heat shock proteins in exercised human skeletal muscle
Open this publication in new window or tab >>Heat shock proteins in exercised human skeletal muscle
2018 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Exercise is considered as an important stressor accompanied by concerted molecular and cellular changes leading to adaptations at the level of skeletal muscle size and function. An important protein family produced by cells in response to stressful conditions is the heat shock proteins (HSPs). It is suggested that the different HSPs play specific roles in acute and longterm responses to exercise-induced stress. The overall aim of this thesis was to explore the expression of four different HSPs (αB-crystallin, HSP27, HSP60 and HSP70) in human skeletal muscle exposed to exercise, with a special emphasis on the role played by HSP27 in the hypertrophy of human skeletal muscle.

One of the major findings was the fibre type-specific expression of HSPs in resting human skeletal muscle, including the preferential expression of HSP27 in fast type II muscle fibres. Another finding was the occurrence of training background-related differences in the expression of HSPs. Also, a cytoplasmic relocation of HSP27, occurring specifically in type II muscle fibres, was shown in response to a single bout of resistance exercise. Interestingly, there were no corresponding changes in response to an endurance exercise bout, suggesting that HSP27 may be specifically involved in the adaptations to resistance exercise. In order to test this hypothesis, an in-vitro exercise model based on the electrical pulse stimulation (EPS) of muscle cells was developed. The EPS protocol, including an 8 h restitution period, induced a significant hypertrophy of muscle cells together with molecular changes similar to those previously described in response to exercise in humans. The role of HSP27 in the hypertrophy of human muscle cells was examined through the downregulation of HSP27. Based on data from morphological and microarray analyses, findings indicate that HSP27 is not mandatory for the hypertrophy of human muscle cells. Overall, the present thesis clarified the expression of different HSPs in human skeletal muscle and provided an in-vitro-based approach for the elucidation of the exact role played by HSPs in the adaptations of human skeletal muscle to exercise.

Place, publisher, year, edition, pages
Örebro: Örebro University, 2018. p. 56
Series
Örebro Studies in Sport Sciences, ISSN 1654-7535 ; 28
Keywords
Endurance training, Resistance training, Muscle Fibre Type, Electrical Pulse Stimulation, Muscle Hypertrophy
National Category
Sport and Fitness Sciences
Identifiers
urn:nbn:se:oru:diva-68028 (URN)978-91-7529-260-1 (ISBN)
Public defence
2018-10-17, Örebro universitet, Gymnastik- och idrottshuset, Hörsal G, Fakultetsgatan 1, Örebro, 09:00 (English)
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Supervisors
Available from: 2018-07-23 Created: 2018-07-23 Last updated: 2018-09-25Bibliographically approved

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Folkesson, MattiasKadi, Fawzi

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