Peritoneal dialysis fluid-induced angiogenesis in rat mesentery is increased by lactate in the presence or absence of glucose
2006 (English)In: ASAIO journal (1992), ISSN 1058-2916, E-ISSN 1538-943X, Vol. 52, no 3, p. 276-281Article in journal (Refereed) Published
Abstract [en]
Angiogenesis may be an important mechanism behind the functional deterioration of the peritoneum leading to ultrafiltration failure in peritoneal dialysis. The present study was designed to compare the angiogenic properties of lactate-, bicarbonate-, and pyruvate-buffered fluids, evaluated separately with and without glucose. Five different fluids (lactate and bicarbonate with and without 2.5% glucose and pyruvate without glucose) were studied for 5 weeks of twice-daily injections in rats. The respective buffers (40 mmol/l) were adjusted to pH 7.2, and sodium, chloride, calcium, and magnesium were present at standard concentrations. The mesenteric window model, based on observation of the translucent peritoneal sections of the small intestine mesentery, was used for immunohistochemical imaging of microvessels (RECA-1 antigen) and macrophages (ED1 and ED2 antigens). All fluids induced angiogenesis as compared with untreated controls. The lactate-buffered fluids induced larger vascularized zones than did their bicarbonate- and pyruvate-buffered counterparts. Angiogenesis was accompanied by a local recruitment of ED1 macrophages from blood. Addition of glucose to the lactate- and bicarbonate-buffered fluids did not seem to alter their pro-angiogenic properties. In conclusion, intraperitoneal exposure to lactate buffer, compared with bicarbonate, stimulates angiogenesis in the presence or absence of glucose.
Place, publisher, year, edition, pages
Lippincott Williams & Wilkins, 2006. Vol. 52, no 3, p. 276-281
Keywords [en]
Antigens, Fluid mechanics, Genetic engineering, Glucose, Immunology, Medical imaging, pH effects, Fluid-induced angiogenesis, Peritoneal dialysis, Ultrafiltration failure, Blood vessel prostheses, antigen, bicarbonate, buffer, calcium, cell antigen, ed 1 antigen, ed 2 antigen, lactic acid, magnesium, peritoneal dialysis fluid, pyruvic acid, reca 1 antigen, sodium chloride, unclassified drug, dialysis fluid, lactic acid derivative, angiogenesis, animal experiment, animal model, animal tissue, article, concentration (parameters), controlled study, diagnostic imaging, imaging system, immunohistochemistry, macrophage, male, mesentery, microvasculature, nonhuman, pH, rat, small intestine, animal, comparative study, drug effect, fluorescence microscopy, methodology, peritoneum macrophage, Sprague Dawley rat, time, vascularization, Animals, Bicarbonates, Buffers, Dialysis Solutions, Hydrogen-Ion Concentration, Lactates, Macrophages, Peritoneal, Microscopy, Fluorescence, Neovascularization, Physiologic, Rats, Rats, Sprague-Dawley, Time Factors
National Category
Pharmacology and Toxicology
Identifiers
URN: urn:nbn:se:oru:diva-85524DOI: 10.1097/01.mat.0000219065.26807.27ISI: 000237835800010PubMedID: 16760716Scopus ID: 2-s2.0-33746687901OAI: oai:DiVA.org:oru-85524DiVA, id: diva2:1465426
2020-09-092020-09-092020-09-09Bibliographically approved