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Expression of Paracrine Effectors in Human Adipose-Derived Mesenchymal Stem Cells Treated With Plasma From Brown Bears (Ursus arctos)
Department of Cardiology, Faculty of Health, Örebro University, Örebro, Sweden.
Örebro University Hospital. Örebro University, School of Medical Sciences. Department of Clinical Research Laboratory.
Örebro University, School of Medical Sciences. Department of Cardiothoracic and Vascular Surgery, Faculty of Medicine and Health, Örebro University, Örebro, Sweden; University Health Care Research Center, Örebro University, Örebro, Sweden. (iRiSC - Inflammatory Response and Infection Susceptibility Centre)ORCID iD: 0000-0003-1785-8540
Örebro University, School of Medical Sciences. Department of Laboratory Medicine.ORCID iD: 0000-0003-2317-5738
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2021 (English)In: Clinical and Translational Science, ISSN 1752-8054, E-ISSN 1752-8062, Vol. 14, no 1, p. 317-325Article in journal (Refereed) Published
Abstract [en]

Adipose-derived mesenchymal stem cells (ADSCs) are promising candidates for novel cell therapeutic applications. Hibernating brown bears sustain tissue integrity and function via unknown mechanisms, which might be plasma borne. We hypothesized that plasma from hibernating bears may increase the expression of favorable factors from human ADSCs. In an experimental study, ADSCs from patients with ischemic heart disease were treated with interventional media containing plasma from hibernating and active bears, respectively, and with control medium. Extracted RNA from the ADSCs was sequenced using next generation sequencing. Statistical analyses of differentially expressed genes were performed using fold change analysis, pathway analysis, and gene ontology. As a result, we found that genes associated with inflammation, such as IGF1, PGF, IL11, and TGFA, were downregulated by > 10-fold in ADSCs treated with winter plasma compared with control. Genes important for cardiovascular development, ADM, ANGPTL4, and APOL3, were upregulated in ADSCs when treated with winter plasma compared with summer plasma. ADSCs treated with bear plasma, regardless if it was from hibernating or active bears, showed downregulation of IGF1, PGF, IL11, INHBA, IER3, and HMOX1 compared with control, suggesting reduced cell growth and differentiation. This can be summarized in the conclusion that plasma from hibernating bears suppresses inflammatory genes and activates genes associated with cardiovascular development in human ADSCs. Identifying the involved regulator(s) holds therapeutic potential.

Place, publisher, year, edition, pages
Wiley-Blackwell Publishing Inc., 2021. Vol. 14, no 1, p. 317-325
National Category
Medical Genetics
Identifiers
URN: urn:nbn:se:oru:diva-85881DOI: 10.1111/cts.12872ISI: 000570678300001PubMedID: 32949228Scopus ID: 2-s2.0-85091017319OAI: oai:DiVA.org:oru-85881DiVA, id: diva2:1470450
Note

Funding Agencies:

Scandinavian Brown Bear Research Project  

Norwegian Institute for Nature Research  

Swedish University of Agricultural Sciences  

Uppsala Genome Center, Sweden  

National Bioinformatics Infrastructure Sweden 

Available from: 2020-09-24 Created: 2020-09-24 Last updated: 2024-01-16Bibliographically approved

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Johansson, KarinKruse, RobertHelenius, GiselaSamano, NinosFröbert, Ole

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