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Activation of NLRP3 by uropathogenic Escherichia coli is associated with IL-1β release and regulation of antimicrobial properties in human neutrophils
Örebro University, School of Medical Sciences. (iRiSC - Inflammatory Response and Infection Susceptibility Centre)
Örebro University, School of Medical Sciences. (iRiSC - Inflammatory Response and Infection Susceptibility Centre)ORCID iD: 0000-0002-9631-2169
Division of Clinical Microbiology, Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet and Karolinska University Hospital, Stockholm, Sweden.
Örebro University, School of Medical Sciences. (iRiSC - Inflammatory Response and Infection Susceptibility Centre)ORCID iD: 0000-0002-4319-7208
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2020 (English)In: Scientific Reports, E-ISSN 2045-2322, Vol. 10, no 1, article id 21837Article in journal (Refereed) Published
Abstract [en]

The NLRP3 inflammasome and IL-1β have recently been linked to the severity of uropathogenic Escherichia coli (UPEC)-mediated urinary tract infection (UTI). However, not much is known about the contribution of NLRP3 to the antimicrobial properties of neutrophils and the release of IL-1β during UPEC infection. The purpose of this study was to elucidate the mechanisms behind UPEC-induced IL-1β release from human neutrophils, and to investigate the contribution of the NLRP3 inflammasome in neutrophil-mediated inhibition of UPEC growth. We found that the UPEC strain CFT073 increased the expression of NLRP3 and increased caspase-1 activation and IL-1β release from human neutrophils. The IL-1β release was mediated by the NLRP3 inflammasome and by serine proteases in an NF-κB-and cathepsin B-dependent manner. The UPEC virulence factors α-hemolysin, type-1 fimbriae and p-fimbriae were all shown to contribute to UPEC mediated IL-1β release from neutrophils. Furthermore, inhibition of caspase-1 and NLRP3 activation increased neutrophil ROS-production, phagocytosis and the ability of neutrophils to suppress UPEC growth. In conclusion, this study demonstrates that UPEC can induce NLRP3 and serine protease-dependent release of IL-1β from human neutrophils and that NLRP3 and caspase-1 can regulate the antimicrobial activity of human neutrophils against UPEC.

Place, publisher, year, edition, pages
Nature Publishing Group, 2020. Vol. 10, no 1, article id 21837
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Microbiology in the medical area
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URN: urn:nbn:se:oru:diva-88120DOI: 10.1038/s41598-020-78651-1ISI: 000600104900004PubMedID: 33318544Scopus ID: 2-s2.0-85098475740OAI: oai:DiVA.org:oru-88120DiVA, id: diva2:1510896
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Örebro University 

Available from: 2020-12-17 Created: 2020-12-17 Last updated: 2024-01-02Bibliographically approved

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Demirel, IsakPersson, AlexanderSärndahl, EvaKruse, RobertPersson, Katarina

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