Carbohydrate restriction following strenuous glycogen-depleting exercise does not potentiate the acute molecular response associated with mitochondrial biogenesis in human skeletal muscleShow others and affiliations
2021 (English)In: European Journal of Applied Physiology, ISSN 1439-6319, E-ISSN 1439-6327, Vol. 121, no 4, p. 1219-1232Article in journal (Refereed) Published
Abstract [en]
PURPOSE: Carbohydrate (CHO) restriction could be a potent metabolic regulator of endurance exercise-induced muscle adaptations. Here, we determined whether post-exercise CHO restriction following strenuous exercise combining continuous cycling exercise (CCE) and sprint interval exercise could affect the gene expression related to mitochondrial biogenesis and oxidative metabolism in human skeletal muscle.
METHODS: In a randomized cross-over design, 8 recreationally active males performed two cycling exercise sessions separated by 4 weeks. Each session consisted of 60-min CCE and six 30-s all-out sprints, which was followed by ingestion of either a CHO or placebo beverage in the post-exercise recovery period. Muscle glycogen concentration and the mRNA levels of several genes related to mitochondrial biogenesis and oxidative metabolism were determined before, immediately after, and at 3 h after exercise.
RESULTS: Compared to pre-exercise, strenuous cycling led to a severe muscle glycogen depletion (> 90%) and induced a large increase in PGC1A and PDK4 mRNA levels (~ 20-fold and ~ 10-fold, respectively) during the acute recovery period in both trials. The abundance of the other transcripts was not changed or was only moderately increased during this period. CHO restriction during the 3-h post-exercise period blunted muscle glycogen resynthesis but did not increase the mRNA levels of genes associated with muscle adaptation to endurance exercise, as compared with abundant post-exercise CHO consumption.
CONCLUSION: CHO restriction after a glycogen-depleting and metabolically-demanding cycling session is not effective for increasing the acute mRNA levels of genes involved in mitochondrial biogenesis and oxidative metabolism in human skeletal muscle.
Place, publisher, year, edition, pages
Springer, 2021. Vol. 121, no 4, p. 1219-1232
Keywords [en]
Endurance exercise, Muscle glycogen, Oxidative metabolism, PGC1A, Sprint interval exercise, Train-low
National Category
Physiology and Anatomy
Identifiers
URN: urn:nbn:se:oru:diva-89507DOI: 10.1007/s00421-021-04594-8ISI: 000616442200001PubMedID: 33564963Scopus ID: 2-s2.0-85100719578OAI: oai:DiVA.org:oru-89507DiVA, id: diva2:1527504
Note
Funding Agency:
Swedish Research Council for Sports Sciences P2017-0138 FO2017-0018 FO2018-0019
2021-02-112021-02-112025-02-10Bibliographically approved