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Sea urchin Paracentrotus lividus immune cells in culture: formulation of the appropriate harvesting and culture media and maintenance conditions
Consiglio Nazionale delle Ricerche, Istituto di Biomedicina e Immunologia Molecolare ‘A. Monroy’, Palermo, Italy.
Consiglio Nazionale delle Ricerche, Istituto di Biomedicina e Immunologia Molecolare ‘A. Monroy’, Palermo, Italy.ORCID iD: 0000-0002-2403-7989
2019 (English)In: Biology open, E-ISSN 2046-6390, Vol. 8, no 3, article id bio039289Article in journal (Refereed) Published
Abstract [en]

The sea urchin is an emergent model system for studying basic and translational immunology. Here we report a new method for the harvesting and maintenance of primary immune cells isolated from adult Paracentrotus lividus, a common Mediterranean sea urchin species. This optimised method uses coelomocyte culture medium, containing a high-affinity Ca2+ chelator, as the ideal harvesting and anti-clotting vehicle and short-term culture medium (≤48 h), and artificial seawater as the master medium that maintains cell survival and in vitro-ex vivo physiological homeostasis over 2 weeks. Gradually reducing the amount of anticoagulant solution in the medium and regularly replacing the medium led to improved culture viability. Access to a robust and straightforward in vitro-ex vivo system will expedite our understanding of deuterostome immunity as well as underscore the potential of sea urchin with respect to biomedicine and regulatory testing.

Place, publisher, year, edition, pages
The Company of Biologists Ltd. , 2019. Vol. 8, no 3, article id bio039289
Keywords [en]
Echinoderm, Cell biology, In vitro-ex vivo model, Immunity, Marine invertebrate, Proxy to human
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:oru:diva-90449DOI: 10.1242/bio.039289ISI: 000466727000012PubMedID: 30718227Scopus ID: 2-s2.0-85062683462OAI: oai:DiVA.org:oru-90449DiVA, id: diva2:1537253
Note

Funding Agency:

European Union's Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant 671881

Available from: 2021-03-15 Created: 2021-03-15 Last updated: 2021-03-17Bibliographically approved

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Alijagic, Andi

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CiteExportLink to record
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Citation style
  • apa
  • ieee
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