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2021 (English)In: Journal of Clinical Medicine, E-ISSN 2077-0383, Vol. 10, no 9, article id 1810Article in journal (Refereed) Published
Abstract [en]
BACKGROUND: To compare two different methods of corneal culture in infectious keratitis: multiple sampling for direct inoculation and enrichment (standard method) and a single sample via transport medium for indirect inoculation (indirect inoculation method).
METHODS: Prospective inclusion of patients fulfilling predefined criteria of infectious keratitis undergoing corneal culture according to both studied methods in a randomized order.
RESULTS: = 0.002) and a significantly higher proportion of microorganisms than the indirect inoculation method, with a Cohen's kappa of 0.38 (95% CI: 0.28-0.49) for agreement between the methods. Subanalysis of culture results showed that direct inoculation on gonococcal agar only combined with the indirect inoculation method resulted in a similar rate of culture positive patients and proportion of detected microorganisms to the standard method.
CONCLUSION: Indirect inoculation of one corneal sample cannot replace direct inoculation of multiple corneal samples without loss of information. A combination of directly and indirectly inoculated samples can reduce the number of corneal samples by four without statistically significant differences in culture outcome or in the proportion of detected microorganisms.
Place, publisher, year, edition, pages
MDPI, 2021
Keywords
Corneal culture, direct inoculation, indirect inoculation, infectious keratitis
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:oru:diva-91681 (URN)10.3390/jcm10091810 (DOI)000650400300001 ()33919274 (PubMedID)2-s2.0-85113191755 (Scopus ID)
Note
Funding Agency:
Region Örebro County Council Research Committee OLL-779911
2021-05-102021-05-102021-12-02Bibliographically approved