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Evaluation of the eazyplex (R) EHEC complete (Amplex) assay for the differentiation of stx1 and stx2 positivity in stool samples
Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, Örebro, Sweden.
Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, Örebro, Sweden.
Department of Clinical Microbiology, Central Hospital, Karlstad, Sweden.
Örebro University, School of Medical Sciences. Örebro University Hospital. Department of Laboratory Medicine, Clinical Microbiology.
2021 (English)In: Journal of Microbiological Methods, ISSN 0167-7012, E-ISSN 1872-8359, Vol. 188, article id 106291Article in journal (Refereed) Published
Abstract [en]

The performance of the eazyplex (R) EHEC complete (Amplex) for the detection of Shiga toxin genes in stool samples was evaluated. The assay performed well in distinguishing between stx1 and stx2 but suboptimal sensitivity may limit its use to complementary testing rather than primary diagnosis of Shiga toxin-producing Escherichia coli infections.
Place, publisher, year, edition, pages
Elsevier, 2021. Vol. 188, article id 106291
Keywords [en]
Loop-mediated amplification, Shiga toxin, Shiga toxin-producing Escherichia coli, Enterohemorrhagic Escherichia coli
National Category
Microbiology in the medical area
Identifiers
URN: urn:nbn:se:oru:diva-94112DOI: 10.1016/j.mimet.2021.106291ISI: 000687891700005PubMedID: 34311019Scopus ID: 2-s2.0-85111498395OAI: oai:DiVA.org:oru-94112DiVA, id: diva2:1591608
Available from: 2021-09-07 Created: 2021-09-07 Last updated: 2023-12-08Bibliographically approved

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Sundqvist, Martin

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