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Green, Black and Rooibos Tea Inhibit Prostaglandin E2 Formation in Human Monocytes by Inhibiting Expression of Enzymes in the Prostaglandin E2 Pathway
Örebro University, School of Medical Sciences. Department of Health Sciences, Karlstad University, Karlstad, Sweden.ORCID iD: 0000-0001-7634-5302
Department of Health Sciences, Karlstad University, Karlstad, Sweden.
Department of Health Sciences, Karlstad University, Karlstad, Sweden; Department of Environmental and Life Sciences/Biology, Karlstad University, Karlstad, Sweden.ORCID iD: 0000-0002-3813-9548
Department of Health Sciences, Karlstad University, Karlstad, Sweden.
2022 (English)In: Molecules, ISSN 1431-5157, E-ISSN 1420-3049, Vol. 27, no 2, article id 397Article in journal (Refereed) Published
Abstract [en]

The formation of prostaglandin E2 (PGE2) is associated with adverse inflammatory effects. However, long-term treatment with nonsteroidal anti-inflammatory drugs (NSAIDs) comes with risk of severe side effects. Therefore, alternative ways to inhibit PGE2 are warranted. We have investigated the effects of tea extracts and the polyphenols epigallocatechin gallate (EGCG) and quercetin on PGE2 formation, determined by immunoassay, and protein expression, determined by immunoblotting, of cytosolic phospholipase A2 (cPLA2), cyclooxygenase 2 (COX-2) and microsomal PGE synthase-1 (mPGES-1) in human monocytes. Green and black tea extracts, and with a lower potency, Rooibos tea extract, inhibited lipopolysaccharide (LPS) and calcium ionophore-induced PGE2 formation. In addition, all tea extracts inhibited the LPS-induced expression of mPGES-1, and the green and black tea extracts also inhibited, to a lesser extent, COX-2 expression. The tea extracts only marginally reduced cPLA2 expression and had no effect on COX-1 expression. EGCG, present in green and black tea, and quercetin, present in all three teas, also inhibited PGE2 formation and expression of mPGES-1, COX-2 and cPLA2. Cell-based and cell-free assays were also performed to evaluate direct effects on the enzymatic activity of COX and PGE synthases. Mainly, the cell-free assay demonstrated partial inhibition by the tea extracts and polyphenols. However, the inhibition required higher doses compared to the effects demonstrated on protein expression. In conclusion, green and black tea, and to a lesser extent Rooibos tea, are potent inhibitors of PGE2 formation in human monocytes, and mediate their effects by inhibiting the expression of the enzymes responsible for PGE2 formation, especially mPGES-1.

Place, publisher, year, edition, pages
MDPI, 2022. Vol. 27, no 2, article id 397
Keywords [en]
green tea, black tea, rooibos tea, epigallocatechin gallate, quercetin, monocytes, microsomal prostaglandin E2 synthase, cyclooxygenase, prostaglandin E2, phospholipase A2
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:oru:diva-96642DOI: 10.3390/molecules27020397ISI: 000747315700001PubMedID: 35056712Scopus ID: 2-s2.0-85122486278OAI: oai:DiVA.org:oru-96642DiVA, id: diva2:1631354
Available from: 2022-01-24 Created: 2022-01-24 Last updated: 2023-08-28Bibliographically approved

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Hedbrant, Alexander

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