Upregulation of PKN1 as a Prognosis Biomarker for Endometrial CancerShow others and affiliations
2022 (English)In: Cancer Control: Journal of the Moffitt Cancer Cente, ISSN 1073-2748, E-ISSN 1526-2359, Vol. 29, article id 10732748221094797Article in journal (Refereed) Published
Abstract [en]
BACKGROUND: Several markers of survival among endometrial cancer (EC) patients have been proposed, namely, the oncoprotein stathmin, RAF kinase inhibitor (RKIP), Cyclin A, GATA-binding protein 3 (GATA3), and growth and differentiation factor-15 (GDF-15). Their elevated expression correlated significantly with a high stage, serous papillary/clear cell subtypes, and aneuploidy. In a previous study, we reported the elevated expression of the serine/threonine protein kinase N1 (PKN1) in cancerous cells. In the present paper, we studied PKN1 expression in EC tissues from a large cohort of patients, to determine whether PKN1 can serve as a marker for the aggressiveness and prognosis of EC, and/or as a marker of survival among EC patients.
METHODS: Tissue samples from EC patients were examined retrospectively for tumor type, tumor size, FIGO stage and grade, depth of invasion in the myometrium, and presence of lymph node metastasis. The PKN1 protein expression in EC cells was assessed by immunohistochemistry. PKN1 mRNA levels were analyzed in publicly available databases, using bioinformatic tools.
RESULTS: We found that expression of PKN1 at the mRNA and proteins levels tended to increase in high-grade EC samples (P = .0001 and P = .06, respectively). In addition, patients with metastatic disease had higher PKN1 mRNA levels (P = .02). Moreover, patients with high PKN1 expression could be characterized by poorer survival.
CONCLUSIONS: We have shown a trend of the higher PKN1 expression levels in EC patients with poor prognosis. Therefore, PKN1 might be considered as a candidate prognostic marker for EC.
Place, publisher, year, edition, pages
Sage Publications, 2022. Vol. 29, article id 10732748221094797
Keywords [en]
PKN1, endometrial cancer, prognostic marker, survival, tumor progression
National Category
Cancer and Oncology
Identifiers
URN: urn:nbn:se:oru:diva-98917DOI: 10.1177/10732748221094797ISI: 000797516900001PubMedID: 35533253Scopus ID: 2-s2.0-85129489856OAI: oai:DiVA.org:oru-98917DiVA, id: diva2:1657265
Funder
Stockholm County Council, 562083Karolinska Institute, 562083
Note
Funding agencies:
Cancer Research Foundation (Radiumhemmets Forskningsfonder) 151202
Research Program 2.2.5.384 of NAS of Ukraine
2022-05-102022-05-102022-05-31Bibliographically approved