Umbilical cord blood DNA methylation in children who later develop type 1 diabetesDepartment of Computer Science, Aalto University, Espoo, Finland.
Turku Bioscience Centre, University of Turku and Åbo Akademi University, Turku, Finland.
Turku Bioscience Centre, University of Turku and Åbo Akademi University, Turku, Finland; InFLAMES Research Flagship Center, University of Turku, Turku, Finland.
Research Centre of Applied and Preventive Cardiovascular Medicine, University of Turku, Turku, Finland; Centre for Population Health Research, University of Turku and Turku University Hospital, Turku, Finland.
Research Centre for Integrative Physiology and Pharmacology, Institute of Biomedicine, University of Turku, Turku, Finland.
Research Centre for Integrative Physiology and Pharmacology, Institute of Biomedicine, University of Turku, Turku, Finland.
Turku Bioscience Centre, University of Turku and Åbo Akademi University, Turku, Finland.
Turku Bioscience Centre, University of Turku and Åbo Akademi University, Turku, Finland.
Institute for Molecular Medicine Finland (FIMM), University of Helsinki, Helsinki, Finland.
Turku Bioscience Centre, University of Turku and Åbo Akademi University, Turku, Finland; InFLAMES Research Flagship Center, University of Turku, Turku, Finland.
Department of Virology, Faculty of Medicine and Biosciences, University of Tampere, Tampere, Finland.
Turku Bioscience Centre, University of Turku and Åbo Akademi University, Turku, Finland; InFLAMES Research Flagship Center, University of Turku, Turku, Finland; Institute of Biomedicine, University of Turku, Turku, Finland.
Immunogenetics Laboratory, Institute of Biomedicine, University of Turku, Turku, Finland.
Pediatric Research Center, Children's Hospital, University of Helsinki and Helsinki University Hospital, Helsinki, Finland; Research Program for Clinical and Molecular Metabolism, Faculty of Medicine, University of Helsinki, Helsinki, Finland; Center for Child Health Research, Tampere University Hospital, Tampere, Finland.
Turku Bioscience Centre, University of Turku and Åbo Akademi University, Turku, Finland.
Department of Pediatrics, PEDEGO Research Unit, Medical Research Center, Oulu University Hospital and University of Oulu, Oulu, Finland.
Department of Computer Science, Aalto University, Espoo, Finland.
Centre for Population Health Research, University of Turku and Turku University Hospital, Turku, Finland; Research Centre for Integrative Physiology and Pharmacology, Institute of Biomedicine, University of Turku, Turku, Finland; Department of Pediatrics, Turku University Hospital, Turku, Finland.
Turku Bioscience Centre, University of Turku and Åbo Akademi University, Turku, Finland; InFLAMES Research Flagship Center, University of Turku, Turku, Finland; Institute of Biomedicine, University of Turku, Turku, Finland.
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2022 (English)In: Diabetologia, ISSN 0012-186X, E-ISSN 1432-0428, Vol. 65, no 9, p. 1534-1540Article in journal (Refereed) Published
Abstract [en]
AIMS/HYPOTHESIS: Distinct DNA methylation patterns have recently been observed to precede type 1 diabetes in whole blood collected from young children. Our aim was to determine whether perinatal DNA methylation is associated with later progression to type 1 diabetes.
METHODS: Reduced representation bisulphite sequencing (RRBS) analysis was performed on umbilical cord blood samples collected within the Finnish Type 1 Diabetes Prediction and Prevention (DIPP) Study. Children later diagnosed with type 1 diabetes and/or who tested positive for multiple islet autoantibodies (n = 43) were compared with control individuals (n = 79) who remained autoantibody-negative throughout the DIPP follow-up until 15 years of age. Potential confounding factors related to the pregnancy and the mother were included in the analysis.
RESULTS: No differences in the umbilical cord blood methylation patterns were observed between the cases and controls at a false discovery rate <0.05.
CONCLUSIONS/INTERPRETATION: Based on our results, differences between children who progress to type 1 diabetes and those who remain healthy throughout childhood are not yet present in the perinatal DNA methylome. However, we cannot exclude the possibility that such differences would be found in a larger dataset.
Place, publisher, year, edition, pages
Springer, 2022. Vol. 65, no 9, p. 1534-1540
Keywords [en]
Bisulphite sequencing, DNA methylation, Epigenomics, Follow-up study, Type 1 diabetes, Umbilical cord blood
National Category
Endocrinology and Diabetes
Identifiers
URN: urn:nbn:se:oru:diva-99651DOI: 10.1007/s00125-022-05726-1ISI: 000812577400001PubMedID: 35716175Scopus ID: 2-s2.0-85132162740OAI: oai:DiVA.org:oru-99651DiVA, id: diva2:1673021
Funder
Novo NordiskAcademy of Finland, 340231 292335 294337 319280 31444 329277 331790 296801 310561 314443 329278 335434 335611EU, European Research Council, 677943EU, Horizon 2020, 675395
Note
Funding agencies:
University of Turku (UTU)
InFLAMES Flagship Programme of the Academy of Finland 337530
Juvenile Diabetes Research Foundation 1-SRA-2016-342-M-R 1-SRA-2019-732-M-B
Finnish Diabetes Foundation
Special Research Funds for University Hospitals in Finland
Business Finland
Sigrid Juselius Foundation
Jane and Aatos Erkko Foundation
Finnish Cancer Foundation
Turku University Central Hospital
Academy of Finland Centre of Excellence in Molecular Systems Immunology and Physiology Research (2012-2017) 250114 292482
European Foundation for the Study of Diabetes
Pediatric Research Foundation
Turku University Hospital Special Governmental Grants
Biocenter Finland
ELIXIR Finland
Turku Doctoral Programme of Molecular Medicine
Finnish Cultural Foundation
Finnish IT center for science
Kyllikki and Uolevi Lehikoinen Foundation
2022-06-202022-06-202022-08-22Bibliographically approved