Needles from various pine species have been used as bio-monitors for air pollution for decades. Dry and wet deposition of pollutants transported via air, as well as their uptake by the roots of the plants contribute to the levels of major and trace elements found in needles. Due to transmission processes, pollutants can be present in remote areas. Thus, a mountain area in the junction of the Lower Inn Valley with the valley Achental (both in Tyrol, Austria) was chosen for needle sampling. Needles of different age, namely young needles, one-, two- and more- year-old needles were taken from mountain pines (Pinus mugo) at three different altitudes from 1400 m to 2000 m. Five to ten trees were chosen a teach sampling site, where the needles of the same age were pooled. Sampling has been performed every July from 2018 to 2022. In addition, soil samples (A-horizon) were collected at the sampling sites. All samples were dried prior to acidic digestion (either microwave assisted or by high pressure asher). Concentrations of elements in the digestion solutions were then determined using quadrupole and high-resolution inductively coupled plasma mass spectrometry. Advanced quality assurance measures were applied to ensure high reliability of the analytical results and its comparability to previously published data. We found that the influence of the altitude was higher on the levels of macro elements than on those of potentially toxic elements. As for other pine species, accumulation of certain elements with needle age was observed. The elemental composition of fresh sprouts showed a high variability from year to year.