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2022 (English)In: Science of the Total Environment, ISSN 0048-9697, E-ISSN 1879-1026, Vol. 835, article id 155377Article in journal (Refereed) Published
Abstract [en]
Exposure to toxic metals alters host response and that leads to disease development. Studies have revealed the effects of metals on microbial physiology, however, the role of metal resistant bacteria on host response to metals is unclear. The hypothesis that xenobiotic interactions between gut microbes and arsenic influence the host physiology and toxicity was assessed in a Caenorhabditis elegans model. The arsenic-resistant Lysinibacillus sphaericus B1CDA was fed to C. elegans to determine the host responses to arsenic in comparison to Escherichia coli OP50 food. L. sphaericus diet extended C. elegans lifespan compared to E. coli diet, with an increased expression of genes involved in lifespan, stress response and immunity (hif-1, hsp-16.2, mtl-2, abf-2, clec-60), as well as reduced fat accumulation. Arsenic-exposed worms fed L. sphaericus also had a longer lifespan than those fed E. coli and had an increased expression of genes involved in cytoprotection, stress resistance (mtl-1, mtl-2) and oxidative stress response (cyp-35A2, isp-1, ctl-2, sod-1), together with a decreased accumulation of reactive oxygen species (ROS). In comparison with E. coli, L. sphaericus B1CDA diet increased C. elegans fitness while detoxifying arsenic induced ROS and extending lifespan.
Place, publisher, year, edition, pages
Elsevier, 2022
Keywords
Arsenate, Arsenite, C. elegans, Lifespan, Lysinibacillus sphaericus, Reactive oxygen species
National Category
Biochemistry and Molecular Biology Environmental Sciences
Identifiers
urn:nbn:se:oru:diva-98800 (URN)10.1016/j.scitotenv.2022.155377 (DOI)000797863000008 ()35460794 (PubMedID)2-s2.0-85129450732 (Scopus ID)
Funder
Knowledge Foundation, 20180027 20170118Örebro University
2022-05-042022-05-042022-12-19Bibliographically approved