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Detection and isolation of intestinal muscle relaxant substances from the root of Taverniera abyssinica A. Rich
Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden; Department of Biochemistry, Addis Ababa University, Addis Ababa, Ethiopia; Department of Biochemistry, Division of Biomedical Sciences, School of medicine, University of Global Health Equity, Kigali, Rwanda.
Örebro University, School of Medical Sciences. Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden; Department of Cardiothoracic and Vascular Surgery, Örebro University, Örebro, Sweden.ORCID iD: 0000-0002-8461-5074
Department of Biochemistry, Addis Ababa University, Addis Ababa, Ethiopia.
Department of Biochemistry, Addis Ababa University, Addis Ababa, Ethiopia.
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2023 (English)In: Journal of Ethnopharmacology, ISSN 0378-8741, E-ISSN 1872-7573, Vol. 312, article id 116498Article in journal (Refereed) Published
Abstract [en]

ETHNOPHARMACOLOGICAL RELEVANCE: In Ethiopian traditional medicine the root of Taverniera abyssinica A.Rich is known as a remedy for sudden gastrointestinal cramping and fever. In this study we have isolated and identified the bioactive principle of Taverniera abyssinica that exerts effects on isolated smooth muscle tissues of the rabbit duodenum and guinea-pig ileum.

AIM OF THE STUDY: To isolate and purify the bioactive principle from the root of Taverniera abyssinica A.Rich by bioassay-guided fractionation, HPLC purification and masspectrometry, with further investigation of its bioactivity on isolated smooth muscle strips.

MATERIALS AND METHODS: Roots of Taverniera abyssinica A.Rich extracted in 75% methanol/water were fractioned with a reverse phase column and then subjected to HPLC purification. Each fraction collected from the HPLC was tested for its bioactivity using electric field stimulation-evoked contractions of the rabbit duodenum and guinea-pig ileum. Finally, detailed structural analysis of the fraction displaying significant bioactivity was made by mass spectrometry.

RESULTS: Through bioassay-guided fractionation and HPLC purification the bioactive fractions were identified. These were tested for bioactivity on isolated smooth muscle strips which showed about 50% inhibition of contractions evoked by electric field stimulation. These compounds were identified as formononetin, afrormosin and tectorigenin by using masspectrometry applying relevant standards for detection.

CONCLUSION: The traditionally claimed smooth muscle-relaxing effect of the roots of Taverniera abyssinica A.Rich is essentially due the three isolated and purified the two isoflavones formononetin, afrormosin as well as the metoxyisoflavone tectorigenin, along with possibly other not yet purified bioactive substances, however with similar smooth muscle-relaxing properties.

Place, publisher, year, edition, pages
Elsevier, 2023. Vol. 312, article id 116498
Keywords [en]
Afrormosin, Bioassay, Formononetin, Muscle strip, Smooth muscle, Tectorigenin
National Category
Physiology
Identifiers
URN: urn:nbn:se:oru:diva-105538DOI: 10.1016/j.jep.2023.116498ISI: 000983637800001PubMedID: 37059252Scopus ID: 2-s2.0-85152664400OAI: oai:DiVA.org:oru-105538DiVA, id: diva2:1751064
Available from: 2023-04-17 Created: 2023-04-17 Last updated: 2023-06-02Bibliographically approved

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Nilsson, Kristofer F.

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