Belimumab concentration measurements using a homologous anti-idiotype immunoassayDivision of Rheumatology, Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden; Department of Gastroenterology, Dermatology and Rheumatology, Karolinska University Hospital, Stockholm, Sweden.
Department of Biomedical and Clinical Sciences, Division of Inflammation and Infection/Rheumatology, Linköping University, Linköping, Sweden.
Department of Clinical Sciences Lund, Rheumatology, Lund University, Skåne University Hospital, Lund, Sweden.
Sanquin Reagents B.V., Amsterdam, the Netherlands.
Biologics Laboratory, Sanquin Diagnostic Services, Amsterdam, the Netherlands.
Biologics Laboratory, Sanquin Diagnostic Services, Amsterdam, the Netherlands.
Biologics Laboratory, Sanquin Diagnostic Services, Amsterdam, the Netherlands.
Biologics Laboratory, Sanquin Diagnostic Services, Amsterdam, the Netherlands.
Department of Rheumatology and Clinical Immunology, Amsterdam Rheumatology and immunology Center, Amsterdam University Medical Center, Amsterdam, the Netherlands; Amsterdam Institute for Infection and Immunity, Amsterdam, the Netherlands.
Department of Rheumatology and Clinical Immunology, Amsterdam Rheumatology and immunology Center, Amsterdam University Medical Center, Amsterdam, the Netherlands; Amsterdam Institute for Infection and Immunity, Amsterdam, the Netherlands.
Amsterdam Rheumatology and immunology Center|Reade, Amsterdam, the Netherlands; Department of Immunopathology, Sanquin Research, Amsterdam, The Netherlands, and Landsteiner Laboratory, Academic Medical Centre, University of Amsterdam, Amsterdam, the Netherlands.
Department of Rheumatology and Clinical Immunology, Amsterdam Rheumatology and immunology Center, Amsterdam University Medical Center, Amsterdam, the Netherlands; Amsterdam Institute for Infection and Immunity, Amsterdam, the Netherlands.
Biologics Laboratory, Sanquin Diagnostic Services, Amsterdam, the Netherlands; Amsterdam Institute for Infection and Immunity, Amsterdam, the Netherlands; Department of Immunopathology, Sanquin Research, Amsterdam, The Netherlands, and Landsteiner Laboratory, Academic Medical Centre, University of Amsterdam, Amsterdam, the Netherlands.
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2024 (English)In: JIM - Journal of Immunological Methods, ISSN 0022-1759, E-ISSN 1872-7905, Vol. 532, article id 113717Article in journal (Refereed) Published
Abstract [en]
Monitoring belimumab concentrations in patients can be a valuable tool for assessing treatment response and for personalizing drug doses. Various assay formats may be used to measure concentrations of therapeutic monoclonal antibodies. A particularly useful format involves the use of anti-idiotype monoclonal antibodies, selected to be highly specific to the antibody of interest. Here, we describe the development of a specific, high-affinity anti-idiotype antibody to belimumab, and the application of this antibody in a homologous sandwich ELISA to measure belimumab concentrations.
Place, publisher, year, edition, pages
Elsevier, 2024. Vol. 532, article id 113717
Keywords [en]
Anti-idiotype antibodies, Belimumab, PK/ADA assays, Rabbit monoclonal antibodies
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:oru:diva-114616DOI: 10.1016/j.jim.2024.113717ISI: 001272244200001PubMedID: 38960066Scopus ID: 2-s2.0-85197474221OAI: oai:DiVA.org:oru-114616DiVA, id: diva2:1881904
Funder
Swedish Rheumatism Association, R-969696King Gustaf V Jubilee Fund, FAI-2020-0741Swedish Society of Medicine, SLS-974449Nyckelfonden, OLL-974804Region Stockholm, FoUI-955483Karolinska Institute
Note
IP is supported by grants from the Swedish Rheumatism Association (R-969696), King Gustaf V's 80-year Foundation (FAI-2020-0741), Swedish Society of Medicine (SLS-974449), Nyckelfonden (OLL-974804), Professor Nanna Svartz Foundation (2021-00436), Ulla and Roland Gustafsson Foundation (2021-26), Region Stockholm (FoUI-955483), and Karolinska Institutet.
2024-07-042024-07-042024-08-12Bibliographically approved