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The expression of vascular endothelial growth factor in skeletal muscle of patients with sleep disorders
Örebro University, School of Health and Medical Sciences. Dalarna University, Falun, Sweden. (Research in musculoskeletal health and disease , RISPA)
Avesta Hospital, Avesta, Sweden.
Örebro University, School of Health and Medical Sciences. (Research in musculoskeletal health and disease)
Örebro University, School of Health and Medical Sciences, Örebro University, Sweden. (RISPA- MEP-RG)ORCID iD: 0000-0002-9831-0896
2009 (English)In: Muscle and Nerve, ISSN 0148-639X, E-ISSN 1097-4598, Vol. 40, no 4, 556-561 p.Article in journal (Refereed) Published
Abstract [en]

An increased capillary network has been observed in the skeletal muscle in patients with Restless Legs Syndrome (RLS) and Obstructive sleep apnea syndrome (OSAS). These changes could be due to upregulation of growth factors responsible for angiogenesis.

The aim of the study was to examine the occurrence and localization of VEGF and capillary proliferation in skeletal muscle of RLS (n=12), OSAS (n=12) and controls (n=11).

Double-immunofluorescence staining for capillaries (CD31) and VEGF, and proliferating cells (Ki-67), was carried out on biopsies taken from the tibialis anterior. The percentage of capillaries expressing VEGF (CD31,VEGF+)  was significantly higher in OSAS and RLS compared to controls. The percentage of proliferating capillaries (CD31,Ki-67+)  was significantly higher in OSAS compared with controls.

In conclusion our study shows the occurrence of proliferation of endothelial cells in skeletal muscle in RLS and OSAS, supporting an upregulation of VEGF located in capillaries probably due to local hypoxia.

Place, publisher, year, edition, pages
New York, NY: John Wiley & Sons , 2009. Vol. 40, no 4, 556-561 p.
Keyword [en]
VEGF, immunofluorescence, capillary, RLS, OSAS
National Category
Medical and Health Sciences Social Sciences Interdisciplinary Sport and Fitness Sciences
Research subject
Sports Science
Identifiers
URN: urn:nbn:se:oru:diva-6213DOI: 10.1002/mus.21357ISI: 000270794900006OAI: oai:DiVA.org:oru-6213DiVA: diva2:211093
Available from: 2009-04-08 Created: 2009-04-08 Last updated: 2016-12-07Bibliographically approved
In thesis
1. Skeletal muscle in Restless legs syndrome (RLS) and Obstructive sleep apnoea syndrome (OSAS)
Open this publication in new window or tab >>Skeletal muscle in Restless legs syndrome (RLS) and Obstructive sleep apnoea syndrome (OSAS)
2009 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Restless legs syndrome (RLS) and Obstructive sleep apnoea syndrome (OSAS) are two sleep disorders that affect daily life with symptoms such as sleepiness and fatigue. It was therefore hypothesised that the skeletal muscle could be affected as symptoms from skeletal muscle are common. The overall aim of the thesis was to investigate aerobic capacity and structure of skeletal muscle in patients with OSAS and RLS and an age matched control group to provide information regarding the underlying mechanisms. The specific aims were to investigate muscle fibre composition, capillary network, capillary proliferation and sings of local inflammation in musculus tibialis anterior of RLS and OSAS.OSAS and RLS patients had a significantly lower predicted VO2 max expressed in ml/min/kg compared with the control group and in the OSAS group apnoes-hyponea index (AHI) was inversely correlated to maximal oxygen uptake Fibre type composition and muscle fibre cross sectional area in the tibialis anterior muscle was equal in all groups with a predominant proportion of slow type I fibres and a smaller fibre area in slow type I fibres compared to fast type II fibres. The distribution of fast fibres (I/IIA, IIA) did not differ except for the group IIX and IIA/IIX where OSAS and RLS had a significantly higher percentage. OSAS patients had a significantly higher number of capillaries per fibre (CAF) for slow type I fibres and CAF per fibre area (CAFA) for fast type II fibres. CFPE- index (capillary to fibre perimeter exchange) and LC/PF-index (length of capillary/perimeter of fibre) were higher in both patient groups. Vascular endothelial growth factor (VEGF) and proliferating endothelial cells were analysed by double-immunofluorescence staining and were presented to a greater extent in the patient groups compared with the healthy controls. Based on normal amounts of T-cells and macrophages in the histological picture it was also demonstrated that local inflammation was not present in the tibialis anterior muscle of RLS and OSAS whish was also supported by the absence of expression of major histocompatibility complex class I molecules (MHC class I) on the surface of the tibialis anterior muscle fibres.In conclusion, the low predicted VO2 max together with higher percentage of type IIX and IIA/IIX muscle fibres indicates a low central capacity in the patient groups. The increased capillary network and the absence of inflammation indicate the occurrence of local hypoxia in tibialis anterior muscle in patients OSAS and RLS.

Place, publisher, year, edition, pages
Örebro: Örebro university, 2009. 61 p.
Series
Örebro Studies in Sport Sciences, ISSN 1654-7535 ; 3
Keyword
RLS, OSAS, aerobic capacity, skeletal muscle fibers, capillary, VEGF
National Category
Dentistry Sport and Fitness Sciences
Research subject
Sports Science
Identifiers
urn:nbn:se:oru:diva-6245 (URN)978-91-7668-661-4 (ISBN)
Public defence
2009-05-08, Hörsal P2, Prismahuset, Örebro Universitet, 13:00 (Swedish)
Opponent
Supervisors
Available from: 2009-04-14 Created: 2009-04-14 Last updated: 2016-06-23Bibliographically approved

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