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Q1U8S3 - a cousin to Majastridin
Örebro University, School of Science and Technology. (Åke Strid)
2009 (English)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

The aim of this work was to determine if the protein Majastridin found in the proteobacterium Rhodobacter blasticus has a functional relative in the hypothetical protein Q1U8S3/ B3XNV1 found in Lactobacillus reuteri. To be able to study the protein, it was overexpressed in  E. coli-cells and purified. As a starting material, the L. reuteri Q1U8S3 gene previously cloned into a pET SUMO vector from Invitrogen was used. The produced protein will be a fusion protein containing a His6-tag, a SUMO-protein and the protein of interest. A nickel column in combination with a gel filtration column was used to purify the protein and after purification, crystallization experiments were set up using standardized kits.

Place, publisher, year, edition, pages
2009. , 19 p.
Keyword [en]
Majastridin, Q1U8S3
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:oru:diva-6693ISRN: ORU-NAT/KEM-GK-2009/0001--SEOAI: oai:DiVA.org:oru-6693DiVA: diva2:216589
Presentation
P2442, Örebro Universitet, Örebro (English)
Uppsok

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Available from: 2009-05-19 Created: 2009-05-11 Last updated: 2009-05-19Bibliographically approved

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CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf