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Visualisation of zebrafish infection by GFP-labelled Vibrio anguillarum
Örebro University, School of Science and Technology.ORCID iD: 0000-0001-7336-6335
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2004 (English)In: Microbial Pathogenesis, ISSN 0882-4010, E-ISSN 1096-1208, Vol. 37, no 1, p. 41-46Article in journal (Refereed) Published
Abstract [en]

Vibrio anguillarum is an invasive pathogen of fish causing a septicaemia called vibriosis. In this work, transparent zebrafish were immersed in water containing green fluorescent protein labelled V. anguillarum. The infection was visualised at the whole fish and single bacterium levels using microscopy. The gastrointestinal tract was the first site where the pathogen was detected. This enteric localisation occurred independently of the flagellum or motility. On the other hand, chemotactic motility was essential for association of the pathogen with the fish surface. In conclusion, the zebrafish infection model provides evidence that the intestine and skin represent sites of infection by V. anguillarum and suggests a host site where chemotaxis may function in virulence.

Place, publisher, year, edition, pages
2004. Vol. 37, no 1, p. 41-46
National Category
Biological Sciences
Research subject
Biology
Identifiers
URN: urn:nbn:se:oru:diva-8840DOI: 10.1016/j.micpath.2004.03.001PubMedID: 15194159OAI: oai:DiVA.org:oru-8840DiVA, id: diva2:281958
Available from: 2009-12-18 Created: 2009-12-18 Last updated: 2017-12-12Bibliographically approved

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Olsson, Per-Erik

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