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Gram-Positive and Gram-Negative Bacteria Induce Different Patterns of Cytokine Production in Human Mononuclear Cells Irrespective of Taxonomic Relatedness
Univ Gothenburg, Inst Biomed, Dept Infect Med Clin Bacteriol, SE-41346 Gothenburg, Sweden.
Univ Gothenburg, Inst Biomed, Dept Infect Med Clin Bacteriol, SE-41346 Gothenburg, Sweden.
Univ Gothenburg, Inst Biomed, Dept Clin Chem & Transfus Med, SE-41346 Gothenburg, Sweden.
Univ Gothenburg, Inst Biomed, Dept Infect Med Clin Bacteriol, SE-41346 Gothenburg, Sweden.
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2010 (English)In: Journal of Interferon and Cytokine Research, ISSN 1079-9907, E-ISSN 1557-7465, Vol. 30, no 1, 23-32 p.Article in journal (Refereed) Published
Abstract [en]

Upon bacterial stimulation, tissue macrophages produce a variety of cytokines that orchestrate the immune response that clears the infection. We have shown that Gram-positives induce higher levels of interleukin-12 (IL-12), interferon-γ (IFN-γ), and tumor necrosis factor (TNF) from human peripheral blood mononuclear cells (PBMCs) than do Gram-negatives, which instead induce more of IL-6, IL-8, and IL-10. Here, we study whether these patterns follows or crosses taxonomic borders. PBMCs from blood donors were incubated with UV-inactivated bacteria representing 37 species from five phyla. IL-12, TNF, IL-1β, IL-6, IL-8, and IL-10 were measured in the supernatants after 24 h and IFN-γ after 5 days. Irrespective of phylogenetic position, Gram-positive bacteria induced much more IL-12 (nine times more on average) and IFN-γ (seven times), more TNF (three times), and slightly more IL-1β (1.5 times) than did Gram-negatives, which instead induced more IL-6 (1.5 times), IL-8 (1.9 times), and IL-10 (3.3 times) than did Gram-positives. A notable exception was the Gram-positive Listeria monocytogenes, which induced very little IL-12, IFN-γ, and TNF. The results confirm the fundamental difference in innate immune responses to Gram-positive and Gram-negative bacteria, which crosses taxonomic borders and probably reflects differences in cell wall structure.

Place, publisher, year, edition, pages
New York, USA: Mary Ann Liebert, 2010. Vol. 30, no 1, 23-32 p.
National Category
Biochemistry and Molecular Biology Cell Biology Immunology
Research subject
Culinary Arts and Meal Science
Identifiers
URN: urn:nbn:se:oru:diva-9722DOI: 10.1089/jir.2009.0033ISI: 000273557300004PubMedID: 20028205Scopus ID: 2-s2.0-75149128993OAI: oai:DiVA.org:oru-9722DiVA: diva2:293712
Available from: 2010-02-12 Created: 2010-02-12 Last updated: 2017-02-20Bibliographically approved

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