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Gram-Positive and Gram-Negative Bacteria Induce Different Patterns of Cytokine Production in Human Mononuclear Cells Irrespective of Taxonomic Relatedness
Inst Biomed, Dept Infect Med Clin Bacteriol,Univ Gothenburg, Gothenburg, Sweden.
Inst Biomed, Dept Infect Med Clin Bacteriol, Univ Gothenburg, Gothenburg, Sweden.
Inst Biomed, Dept Clin Chem & Transfus Med, Univ Gothenburg, Gothenburg, Sweden.
Inst Biomed, Dept Infect Med Clin Bacteriol, Univ Gothenburg, Gothenburg, Sweden.
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2010 (English)In: Journal of Interferon and Cytokine Research, ISSN 1079-9907, E-ISSN 1557-7465, Vol. 30, no 1, p. 23-32Article in journal (Refereed) Published
Abstract [en]

Upon bacterial stimulation, tissue macrophages produce a variety of cytokines that orchestrate the immune response that clears the infection. We have shown that Gram-positives induce higher levels of interleukin-12 (IL-12), interferon-γ (IFN-γ), and tumor necrosis factor (TNF) from human peripheral blood mononuclear cells (PBMCs) than do Gram-negatives, which instead induce more of IL-6, IL-8, and IL-10. Here, we study whether these patterns follows or crosses taxonomic borders. PBMCs from blood donors were incubated with UV-inactivated bacteria representing 37 species from five phyla. IL-12, TNF, IL-1β, IL-6, IL-8, and IL-10 were measured in the supernatants after 24 h and IFN-γ after 5 days. Irrespective of phylogenetic position, Gram-positive bacteria induced much more IL-12 (nine times more on average) and IFN-γ (seven times), more TNF (three times), and slightly more IL-1β (1.5 times) than did Gram-negatives, which instead induced more IL-6 (1.5 times), IL-8 (1.9 times), and IL-10 (3.3 times) than did Gram-positives. A notable exception was the Gram-positive Listeria monocytogenes, which induced very little IL-12, IFN-γ, and TNF. The results confirm the fundamental difference in innate immune responses to Gram-positive and Gram-negative bacteria, which crosses taxonomic borders and probably reflects differences in cell wall structure.

Place, publisher, year, edition, pages
New York, USA: Mary Ann Liebert, 2010. Vol. 30, no 1, p. 23-32
National Category
Biochemistry and Molecular Biology Cell Biology Immunology
Research subject
Culinary Arts and Meal Science
Identifiers
URN: urn:nbn:se:oru:diva-9722DOI: 10.1089/jir.2009.0033ISI: 000273557300004PubMedID: 20028205Scopus ID: 2-s2.0-75149128993OAI: oai:DiVA.org:oru-9722DiVA, id: diva2:293712
Available from: 2010-02-12 Created: 2010-02-12 Last updated: 2018-04-19Bibliographically approved

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Tham, Wilhelm

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