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Alanine transport in fibroblasts from patients with Bipolar type 1 disorder and controls
Örebro University, School of Health and Medical Sciences.
2010 (English)Independent thesis Basic level (professional degree), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

Background: Bipolar disorder (BD) is a chronic psychiatric disorder that disturbs cognition and behavior and patients with the disorder have an increased risk of suicide or suicide ideation. A possible cause of BD is a dysfunction of the neurotransmitters. Tyrosine is the precursor of the neurotransmitter dopamine and a decreased transport of tyrosine to the brain might lead to decreased dopaminergic activity. A decreased transport of tyrosine across fibroblast cell membranes has previously been found in patients with BD. This implies that the amount of the main tyrosine transporter (LAT1) is less in this group of patients. Since alanine is also transported through this transporter its transport might also be affected. The aim of this study was thus to investigate the alanine transport in fibroblasts from patients with BD type-1 and controls and to see if there was any difference in this transport between patients and controls.

Subjects and methods: Fibroblast cells from 7 patients with BD type-1, fulfilling the DMS-IV diagnostic criteria and from 6 controls were used in this study. The transport of alanine was studied by the use of the cluster tray method. The kinetic parameters maximal transport capacity (Vmax) and affinity constant (Km) of alanine transport were determined from a Lineweaver-Burk plot.

Results: No significant differences in the kinetic parameters (Vmax and Km) of alanine transport were observed in fibroblasts from patients with BD in comparison to controls. However, there was a slight decrease in the Vmax for patients with BD (p=0.090).

Conclusion: The results indicate that the alanine transport might not be aberrant in patients with BD. However, power analysis on the Vmax data showed that if increasing sample size (n>30) a statistical significant difference between patients and controls might be observed, if the trend is sustained. Thus, further studies, including larger sample groups, are needed in order to validate these findings.

Place, publisher, year, edition, pages
2010. , p. 25
Keyword [en]
Alanine transport; Bipolar disorder; Fibroblasts; Tyrosine transport
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:oru:diva-11076ISRN: ORU-HAK/BML-GY-2010/0005--SEOAI: oai:DiVA.org:oru-11076DiVA: diva2:324633
Presentation
2010-05-27, Forum huset, Örebro University, Örebro, 11:00 (English)
Uppsok
Medicine
Supervisors
Examiners
Available from: 2010-10-15 Created: 2010-06-15 Last updated: 2017-10-18Bibliographically approved

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CiteExportLink to record
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