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Pyrosequencing of the DNA gyrase gene in Neisseria species: effective indicator of ciprofloxacin resistance in Neisseria gonorrhoeae
Örebro University, Department of Clinical Medicine.
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2006 (English)In: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), ISSN 0903-4641, E-ISSN 1600-0463, Vol. 114, no 12, 837-841 p.Article in journal (Refereed) Published
Abstract [en]

The quinolone resistance determining region (QRDR) of the gyrA gene in ciprofloxacin-susceptible strains (n=53) and strains of Neisseria spp. with reduced susceptibility (n=70) was determined by the pyrosequencing method. Results showed that the QRDR of the gyrA gene is an effective molecular indicator of resistance to ciprofloxacin in Neisseria gonorrhoeae, and presumably in Neisseria meningitidis, but not in all other Neisseria spp. This sequence was not unique for N. gonorrhoeae and seems unsuitable for species verification of N. gonorrhoeae. However, whether it is also possible to use this region for verification depends on the specificity of the primary screening method used.

Place, publisher, year, edition, pages
2006. Vol. 114, no 12, 837-841 p.
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Medical and Health Sciences Microbiology in the medical area
Research subject
Microbiology
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URN: urn:nbn:se:oru:diva-12044DOI: 10.1111/j.1600-0463.2006.apm_495.xPubMedID: 17207083OAI: oai:DiVA.org:oru-12044DiVA: diva2:355113
Available from: 2010-10-05 Created: 2010-10-05 Last updated: 2017-12-12Bibliographically approved

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Unemo, MagnusFredlund, Hans

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