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Evaluation of antibacterial efficacy of photo-activated riboflavin using ultraviolet light (UVA)
Örebro University, School of Health and Medical Sciences.
2010 (English)In: Graefe's Archives for Clinical and Experimental Ophthalmology, ISSN 0721-832X, E-ISSN 1435-702X, Vol. 248, no 2, p. 207-212Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: To evaluate the antibacterial efficacy of photo-activated riboflavin using Ultraviolet A (UVA) on three bacterial strains commonly detected in keratitis. METHODS: Three bacterial strains (Staphylococcus epidermidis, Staphylococcus aureus and Pseudomonas aeruginosa) were cultured on blood/hematin-agar plates and dispersed in PBS. Dispersion was done of 10 microl of bacterial stock-solutions in 90 microl of RPMI, where different riboflavin molarities had been added, to achieve a bacterial concentration of 1-4 x 10 (4)/ml. Riboflavin end molarities before illumination were 0, 100, 200, 300 and 400 microM. Each solution had a negative control. The solutions were illuminated with UVA (365 nm) for 30 minutes (5.4 J/cm(2)) and then continued for a total time of 60 minutes (10.8 J/cm(2)). A count of CFU was conducted after incubation and results compared. RESULTS: In all tested strains, a slight decrease of bacteria was seen when exposed to UV for 30 minutes. A doubling of the UV dose showed a marked decrease of bacterial count in all bacteria tested. The combination of UV and riboflavin showed a more extensive reduction of CFU, confirming an interaction effect between UV and riboflavin. CONCLUSION: Riboflavin photo-activation using UVA (365 nm) can achieve an extensive eradication of bacteria, and the combination is more potent in reducing bacterial number than UV alone.

Place, publisher, year, edition, pages
2010. Vol. 248, no 2, p. 207-212
National Category
Medical and Health Sciences
Research subject
Medicine
Identifiers
URN: urn:nbn:se:oru:diva-12083DOI: 10.1007/s00417-009-1231-2ISI: 000273313100008PubMedID: 19921518OAI: oai:DiVA.org:oru-12083DiVA, id: diva2:355164
Available from: 2010-10-05 Created: 2010-10-05 Last updated: 2018-02-27Bibliographically approved
In thesis
1. Ultraviolet light A (UVA) photoactivation of riboflavin as a potential therapy for infectious keratitis
Open this publication in new window or tab >>Ultraviolet light A (UVA) photoactivation of riboflavin as a potential therapy for infectious keratitis
2011 (English)Doctoral thesis, comprehensive summary (Other academic)
Place, publisher, year, edition, pages
Örebro: Örebro universitet, 2011. p. 70
Series
Örebro Studies in Medicine, ISSN 1652-4063 ; 63
National Category
Medical and Health Sciences Surgery
Research subject
Surgery
Identifiers
urn:nbn:se:oru:diva-20130 (URN)978-91-7668-834-2 (ISBN)
Public defence
2011-12-16, Wilandersalen, Universitetssjukhuset, Örebro, 09:15 (Swedish)
Opponent
Supervisors
Available from: 2011-10-20 Created: 2011-10-20 Last updated: 2017-10-17Bibliographically approved

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Makdoumi, KarimCrafoord, Sven

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