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Zebrafish feminization in response to heat killed bacterial exposure suggests a function for anti-apoptotic genes in oocyte maintenance
Örebro University, School of Science and Technology. (Örebro Life Science Center)
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(English)Manuscript (preprint) (Other academic)
National Category
Natural Sciences Biological Sciences
Research subject
Biology
Identifiers
URN: urn:nbn:se:oru:diva-12238OAI: oai:DiVA.org:oru-12238DiVA, id: diva2:357407
Available from: 2010-10-18 Created: 2010-10-18 Last updated: 2017-10-17Bibliographically approved
In thesis
1. Characterization and environmental influences on inflammatory and physiological responses
Open this publication in new window or tab >>Characterization and environmental influences on inflammatory and physiological responses
2010 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Pharmaceuticals are regularly released into the environment, in particular nonsteroidalanti-inflammatory drugs (NSAIDs) and antibiotics. The measuredconcentrations are relatively low and have therefore been considered to be harmless.However, several pharmaceuticals, including naproxen and atenolol, are stable for upto 1 year in the environment, which increases the risk for accumulation. Evaluation ofthe effects of pharmaceuticals on induced inflammatory responses is thereforenecessary for the assessment of potential risks. Since NF-κB and MAPK are the mainpathways known to be critical regulators of inflammatory responses, intracellularsignalling and effects on these systems were examined in vitro using human cell-lines.NSAIDs were shown to significantly reduce NF-κB activity at environmentallyrelevant concentrations. Suppression of immune responses may lead to progressiveinfections since inflammatory responses are controlled by a cooperative activity ofAP-1 and NF-κB. Alterations in the activity of transcription factors and proinflammatorycytokine and chemokine levels such as TNF, IL-6 and CXCL8 areassociated with several human diseases including cystic fibrosis and AIDS. PMAexposure resulted in a rapid NF-κB activation, while extended treatment suppressedNF-κB and activated AP-1. Suppression of NF-κB activity may be due to PKCdependentBcl10 degradation, which decreased in response to PMA and correlatedwith the NF-κB activity. Regulation of cytokine expression revealed that NF-κB wasessential for IL-6 but not CXCL8 expression following specific inhibition of NF-κB,without affecting AP-1 activity. Furthermore, several reports have indicated theimportance of a functional NF-κB complex in zebrafish embryogenesis, whereblockage of NF-κB activation resulted in a deformation of the tail. Our results indicatea suppression of apoptotic pathways following activation of inflammatory mediatorsin response to HK E. coli treatment. These signals acted to direct zebrafish sexdifferentiation towards feminization. NF-κB was shown to regulate zp2 geneexpression, an indicator of oocyte development. Zebrafish sex determination was alsoshown to start early, prior to 16 days post fertilization. The results support thetransition through a juvenile ovary stage and suggests that steriodogenesis is aconsequence of sex differentiation rather than a regulatory mechanism.Control of prescription, use and disposal of pharmaceuticals is therefore importantto preserve human health, biotic processes and to avoid developmental alterations inaquatic organisms. The complexity of regulatory systems involved in inflammationsuggest that there is a need to further evaluate the signalling pathways involved inorder to provide a better understanding of cellular responses to manmade substances,but also to offer an insight into possible development of alternative treatments forhuman diseases with elevated cytokine/chemokine levels.

Place, publisher, year, edition, pages
Örebro: Örebro universitet, 2010. p. 53
Series
Örebro Studies in Life Science ; 7
Keywords
Inflammation, NF-κB, cytokines, pharmaceuticals, zebrafish
National Category
Biological Sciences Natural Sciences
Research subject
Biology
Identifiers
urn:nbn:se:oru:diva-11251 (URN)978-91-7668-760-4 (ISBN)
Public defence
2010-11-19, Hörsal M Musikskolan, Örebro universitet, Fakultetsgatan 1, Örebro, 10:15
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Note
Note: The article "Involvement of NF-κB and AP-1 in the regulation of IL-6 and CXCL8 in Jurkat T-cells." on page 31 in the disseration is published in BMC Immunology with the new name "Differential cytokine regulation by NF-κB and AP-1 in Jurkat T-cells"Available from: 2010-06-24 Created: 2010-06-24 Last updated: 2017-10-18Bibliographically approved

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Khalaf, HazemOlsson, Per-Erik

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