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CFTR and tight junctions in cultured bronchial epithelial cells
Örebro University, School of Health and Medical Sciences.
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2010 (English)In: Experimental and molecular pathology (Print), ISSN 0014-4800, E-ISSN 1096-0945, Vol. 88, no 1, p. 118-127Article in journal (Refereed) Published
Abstract [en]

Airway epithelial salt and water transport takes place through paracellular and transcellular pathways. This transport depends critically on the epithelial sodium channel (ENaC) and the cystic fibrosis transmembrane conductance regulator (CFTR), operating in concert with the paracellular pathway through the tight junctions (TJ). Normal (16HBE14o-), cystic fibrosis (CFBE41o-), and corrected CFBE41o- (CFBE41o-pCep4 overexpressing wtCFTR) airway epithelial cell lines were cultured under isotonic conditions. Transepithelial electrical resistance (TEER) was measured as indicator of the tightness of the cultures. Morphology was investigated by immunofluorescence and paracellular permeability by lanthanum nitrate or [C-14] mannitol as permeability markers. The CFTR-defective cell line CFBE41o- developed higher TEER than its corrected counterpart CFBE41o-pCep4. Addition of a specific inhibitor of CFTR (CFTRinh-172) to 16HBE14o- and CFBE41o-pCep4 cells resulted in a time-dependent increase in TEER, whereas stimulation of CFTR by IBMX and forskolin caused a decrease. Permeability to lanthanum and [C-14] mannitol was lower in CFBE41o- and in 16HBE14o- cells exposed to CFTRinh-172, compared to untreated 16HBE14o- and CFBE41o-pCep4 cells, respectively. 16HBE14o- cells exposed to IBMX and forskolin showed higher permeability to lanthanum but lower permeability to [C-14] mannitol compared to control. Immunofluorescence revealed a disorganization of F-actin and alpha-tubulin in 16HBE14o- cells and CFBE41o- pCep4 exposed to CFTRinh-172 and in CFBE41o- cells. Changes in F-actin and alpha-tubulin in 16HBE14o- cells exposed to IBMX and forskolin were also seen. These results suggest the possibility of an interaction between CFTR and the TJ protein complex, probably via the cytoskeleton. (C) 2009 Elsevier Inc. All rights reserved.

Place, publisher, year, edition, pages
2010. Vol. 88, no 1, p. 118-127
Keyword [en]
CFTR, Transepithelial electrical resistance, Tight junctions, Cytoskeleton
National Category
Medical and Health Sciences
Research subject
Medicine
Identifiers
URN: urn:nbn:se:oru:diva-13020DOI: 10.1016/j.yexmp.2009.09.018ISI: 000274500100017OAI: oai:DiVA.org:oru-13020DiVA, id: diva2:382774
Available from: 2011-01-03 Created: 2011-01-03 Last updated: 2017-12-11Bibliographically approved

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Johannesson, MarieRoomans, Godfried M.

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