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Butyrate enemas do not affect human colonic MUC2 and TFF3 expression
Dept Internal Med, Div Gastroenterol Hepatol, Nutrim, Maastricht Univ, Maastricht, Netherlands; TI Food & Nutr, Wageningen, Netherlands.
Dept Internal Med, Div Gastroenterol Hepatol, Nutrim, Maastricht Univ, Maastricht, Netherlands; TI Food & Nutr, Wageningen, Netherlands.
Dept Pediat, Div Neonatol, Erasmus MC, Rotterdam, Netherlands; Sophia Childrens Univ Hosp, Rotterdam, Netherlands.
Dept Internal Med, Div Gastroenterol Hepatol, Nutrim, Maastricht Univ, Maastricht, Netherlands; TI Food & Nutr, Wageningen, Netherlands.
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2010 (English)In: European Journal of Gastroenterology and Hepathology, ISSN 0954-691X, E-ISSN 1473-5687, Vol. 22, no 9, p. 1134-1140Article in journal (Refereed) Published
Abstract [en]

Introduction The colonic mucus layer plays an important role in the protection of the intestinal epithelium and mainly consists of mucin glycoproteins (primarily MUC2 in the colon) trefoil factor 3 (TFF3) and secretory IgA. Butyrate is a major end product of fermentation of dietary fibres and is associated with beneficial effects on colonic health. Earlier in-vitro and animal studies showed that butyrate modulates MUC2 and TFF3 expression and mucin secretion, although data from human studies are not yet available. Methods Sixteen healthy volunteers and 35 ulcerative colitis (UC) patients in clinical remission self-administered a 60 ml rectal enema containing 100 mmol/l butyrate or placebo once daily for 2 and 3 weeks, respectively. After each treatment, biopsies were taken from the distal sigmoid for quantitative RT-PCR and immunohistochemical analysis of MUC2 and TFF3. In addition, mucosal sections were stained with high iron diamine-alcian blue to distinguish between sialomucins and sulphomucins. To analyse total mucin secretion and secretory IgA concentrations, 24 h faeces were collected during the day before the endoscopic examination. Results The butyrate intervention did not significantly modulate the expression of MUC2 ( fold change: 1.04 and 1.05 in healthy volunteers and ulcerative colitis patients, respectively) or TFF3 (fold change: 0.91 and 0.94 in healthy volunteers and UC patients, respectively). Furthermore, the percentage of sialomucins, mucus secretion and secretory IgA concentrations were not affected by the butyrate intervention in both the groups. Conclusion Butyrate exposure in healthy volunteers and UC patients in remission did not affect the measured parameters of the colonic mucus layer. Eur J Gastroenterol Hepatol 22: 1134-1140 (C) 2010 Wolters Kluwer Health | Lippincott Williams & Wilkins.

Place, publisher, year, edition, pages
2010. Vol. 22, no 9, p. 1134-1140
Keywords [en]
butyrate, MUC2, mucin, secretory IgA, trefoil factor, ulcerative colitis
National Category
Medical and Health Sciences
Research subject
Medicine
Identifiers
URN: urn:nbn:se:oru:diva-12902DOI: 10.1097/MEG.0b013e32833a6ca0ISI: 000280604500015OAI: oai:DiVA.org:oru-12902DiVA, id: diva2:383435
Available from: 2011-01-05 Created: 2011-01-03 Last updated: 2018-04-23Bibliographically approved

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