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The effect of nitric oxide on adherence of P-fimbriated uropathogenic Escherichia coli to human renal epithelial cells
Sch Pure & Appl Nat Sci, Univ Kalmar, Kalmar, Sweden.
Univ Kalmar, Sch Pure & Appl Nat Sci, SE-39182 Kalmar, Sweden.
Örebro University, School of Health and Medical Sciences. Sch Pure & Appl Nat Sci, Univ Kalmar, Kalmar, Sweden.
2010 (English)In: BJU International, ISSN 1464-4096, E-ISSN 1464-410X, Vol. 105, no 12, p. 1726-1731Article in journal (Refereed) Published
Abstract [en]

Objectives: To examine the effect of nitric oxide (NO), an endogenous component of the host defence in urinary tract infection, on the adherence of P-fimbriated uropathogenic Escherichia coli (UPEC) to human renal epithelial cells.

Materials and Methods: Two wild-type UPEC strains (AD110 and IA2) and the P-fimbriated recombinant strain HB101pPIL-75 were used. Bacteria were allowed to adhere to the human renal epithelial cell line A498 and attachment was evaluated in the absence or presence of the NO donor DETA/NONOate (1 mm). Total RNA was extracted from NO-exposed bacteria in static urine cultures, followed by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis of the papG gene that encodes the P-fimbriae adhesin PapG.

Results: Bacterial adherence to A498 cells was fimbriae-dependent and the ability to agglutinate human P-1 positive erythrocytes confirmed that the used strains were P-fimbriated. UPEC strains AD110 and IA2 attached by a mean of 8 bacteria/cell and 20 bacteria/cell, respectively. In the presence of DETA/NONOate, the attachment of AD110 and IA2 to A498 cells was significantly reduced by a mean (sem) of 34 (3.9)% and 45 (14)%, respectively. The expression of papG was decreased after DETA/NONOate exposure as shown by semiquantitative RT-PCR.

Conclusion: NO disrupted functional adhesion of P-fimbriated UPEC to kidney epithelial cells, suggesting that NO-production from epithelial cells in the urinary tract may limit bacterial colonization at the mucosal surface. The reduced adherence may involve transcriptional effects of NO on papG expression, but further studies are needed to establish the underlying mechanisms.

Place, publisher, year, edition, pages
Malden, USA: Wiley-Blackwell, 2010. Vol. 105, no 12, p. 1726-1731
Keyword [en]
Nitric oxide, uropathogenic E, coli, renal epithelial cells, papG, adhesion
National Category
Medical and Health Sciences Urology and Nephrology
Research subject
Biomedicine
Identifiers
URN: urn:nbn:se:oru:diva-13466DOI: 10.1111/j.1464-410X.2009.08986.xISI: 000278033700020PubMedID: 19863519Scopus ID: 2-s2.0-77952862001OAI: oai:DiVA.org:oru-13466DiVA, id: diva2:386081
Available from: 2011-01-12 Created: 2011-01-12 Last updated: 2018-04-23Bibliographically approved

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Persson, Katarina

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