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A rapid one-tube PCR method for simultaneously differentiating homozygotes and heterozygotes of the Sp1 binding site polymorphism in collagen type I alpha 1
Örebro University, Department of Clinical Medicine.
2007 (English)In: Molecular and Cellular Probes, ISSN 1044-7431, E-ISSN 1095-9327, Vol. 21, no 3, p. 239-241Article in journal (Refereed) Published
Abstract [en]

Rapid detection of single-base changes is fundamental to molecular medicine. PCR amplification of specific alleles (PASA) has previously been used as a rapid method of genotyping single-nucleotide changes, but one reaction is required for each allele. This paper describes a Bidirectional PASA (Bi-PASA) method, which was developed to distinguish between homozygotes and heterozygotes in one PCR reaction. The method is tested using the Sp1 polymorphism in Collagen type la I. The results demonstrate that Bi-PASA is a simple and rapid method for detecting the zygosity of the polymorphism in a single PCR reaction. (c) 2006 Elsevier Ltd. All rights reserved.

Place, publisher, year, edition, pages
2007. Vol. 21, no 3, p. 239-241
Keywords [en]
collagen type I alpha 1, Sp1 polymorphism, Bi-PASA
National Category
Medical and Health Sciences
Research subject
Medicine
Identifiers
URN: urn:nbn:se:oru:diva-13863DOI: 10.1016/j.mcp.2006.10.007ISI: 000245670800012OAI: oai:DiVA.org:oru-13863DiVA, id: diva2:389863
Available from: 2011-01-20 Created: 2011-01-13 Last updated: 2017-12-11Bibliographically approved

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Lundberg, Gunilla A.

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