oru.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Comparison of Staphylococcus epidermidis isolated from prosthetic joint infections commensal isolates in regards to antibiotic susceptibility, agr type, biofilm production, and epidemiology
Örebro University, School of Health and Medical Sciences.
(English)Manuscript (preprint) (Other academic)
National Category
Medical and Health Sciences Infectious Medicine
Research subject
Infectious Diseases
Identifiers
URN: urn:nbn:se:oru:diva-15251OAI: oai:DiVA.org:oru-15251DiVA, id: diva2:410315
Available from: 2011-04-13 Created: 2011-04-13 Last updated: 2017-10-17Bibliographically approved
In thesis
1. Genotypic and phenotypic characterisation of Staphylococcus epidermidis isolated from prosthetic joint infections
Open this publication in new window or tab >>Genotypic and phenotypic characterisation of Staphylococcus epidermidis isolated from prosthetic joint infections
2011 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Staphylococcus epidermidis has emerged in recent years as an important nosocomial pathogen, especially in infections associated with implanted foreign body materials (e.g., prosthetic joints and heart valves) and in individuals with a compromised immune system (e.g., cancer patients and neonates). Although rare, implant infections are long lasting and cause severe suffering for the patient that includes pain and disability and even increased mortality. One aim of the present thesis was to develop and evaluate a genetic method for species identification and simultaneous detection of rifampicin resistance in staphylococci. A second aim was to examine S. epidermidis isolated from prosthetic joint infections (PJIs) and from wrists and nares of healthy individuals regarding their antibiotic susceptibility, biofilm production, virulence factors, and epidemiology. Comparison with phenotypic diagnostics revealed that 8 (16%) of 49 isolates differed in their species identification in favour of the genetic method. In addition, mutations associated with rifampicin resistance, including two not previously reported, were possible to detect in all isolates resistant to rifampicin. Antibiotic susceptibility testing of 61 PJI isolates showed multi-drug resistance in 91%. Furthermore, the results of the synergy testing revealed that no antibiotic combination was significantly better than the others. Hence, the effects that were possible to detect were isolate dependent. To find a method for discriminating between invasive (n=61) and commensal (n=24) isolates of S. epidermidis genotypic and phenotypic characterisations of biofilm production (including the ica and aap genes), antibiotic susceptibility, virulence-related genes (such as agr and ACME) and epidemiology were performed (using multilocus sequence typing [MLST], typing of the staphylococcal chromosome cassette mec [SCCmec] and PhenePlate). Significant differences were found in antibiotic susceptibility, i.e. there was more resistance among invasive isolates. MLST sequence types (ST) ST2 and ST215 dominated the invasive isolates.

Place, publisher, year, edition, pages
Örebro: Örebro universitet, 2011. p. 117
Series
Örebro Studies in Medicine, ISSN 1652-4063 ; 53
Keyword
Staphylococcus epidermidis, prosthetic joint infections, antibiotic susceptibility, virulence factors, epidemiology, MLST, agr, SCCmec
National Category
Medical and Health Sciences
Research subject
Biomedicine
Identifiers
urn:nbn:se:oru:diva-15171 (URN)978-91-7668-793-2 (ISBN)
Public defence
2011-05-13, Wilandersalen, Universitetssjukhuset, Örebro, 09:00
Opponent
Supervisors
Available from: 2011-04-01 Created: 2011-04-01 Last updated: 2017-10-17Bibliographically approved

Open Access in DiVA

No full text in DiVA

Authority records BETA

Hellmark, Bengt

Search in DiVA

By author/editor
Hellmark, Bengt
By organisation
School of Health and Medical Sciences
Medical and Health SciencesInfectious Medicine

Search outside of DiVA

GoogleGoogle Scholar

urn-nbn

Altmetric score

urn-nbn
Total: 45 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf