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A rapid method for screening of the Stockholm Convention POPs in small amounts of human plasma using SPE and HRGC/HRMS
Örebro University, School of Science and Technology.ORCID iD: 0000-0001-5752-4196
Örebro University, School of Science and Technology.ORCID iD: 0000-0002-4959-2807
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2012 (English)In: Chemosphere, ISSN 0045-6535, E-ISSN 1879-1298, Vol. 86, no 7, p. 747-753Article in journal (Refereed) Published
Abstract [en]

A rapid analytical screening method allowing simultaneous analysis of 23 persistent organic pollutants (POPs) in human plasma was developed. Sample preparation based on solid-phase extraction (SPE) with additional clean-up using small multilayer silica gel columns. SPE was performed using a custom made polystyrene-divinylbenzene sorbent for the extraction of chlorinated and brominated POPs. Special efforts to reduce sample volume and improve speed and efficiency of the analytical procedure were made. Determination of 16 polychlorinated biphenyls (PCBs), 5 organochlorine (OC) pesticides, octachlorinated dibenzo-p-dioxin (OCDD) and polybrominated diphenyl ether (BDE #47) in 0.5 mL human plasma was performed by using high resolution gas chromatography coupled to high resolution mass spectrometry (HRGC/HRMS). Recovery of POPs ranged between 46% and 110%, and reproducibility was below 25% relative standard deviation (RSD) for all target compounds, except for trans-nonachlor and OCDD, which were present only at low levels. Limits of detection (LOD) were for the PCBs between 0.8 and 117.7 pg mL(-1) plasma and for the OC pesticides between 5.9 and 89.1 pg mL(-1) plasma. The LOD for OCDD and BDE #47 were 1.4 pg mL(-1) plasma, and 9.2 pg mL(-1) plasma, respectively. The presented method was successfully applied to 1016 human plasma samples from an epidemiological study on cardiovascular disease. (C) 2011 Elsevier Ltd. All rights reserved.

Place, publisher, year, edition, pages
Elsevier, 2012. Vol. 86, no 7, p. 747-753
Keywords [en]
Persistent organic pollutants, Sample extraction, Human plasma, HRGC/HRMS, Polychlorinated biphenyls, Organochlorine pesticides
National Category
Chemical Sciences
Research subject
Environmental Chemistry
Identifiers
URN: urn:nbn:se:oru:diva-22315DOI: 10.1016/j.chemosphere.2011.11.006ISI: 000301166700008PubMedID: 22153485Scopus ID: 2-s2.0-84856031844OAI: oai:DiVA.org:oru-22315DiVA, id: diva2:513401
Available from: 2012-04-02 Created: 2012-04-02 Last updated: 2017-12-07Bibliographically approved
In thesis
1. Development and application of high-throughput methods for analysis of persistent organic pollutants in human blood
Open this publication in new window or tab >>Development and application of high-throughput methods for analysis of persistent organic pollutants in human blood
2013 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The general public is continuously exposed to a wide range of environmental pollutants. This thesis focuses on a group of anthropogenic chemicals referred to as persistent organic pollutants (POPs) that have been linked to various adverse health effects in humans.

The main objective of this thesis was focused on the development and application of high-throughput methods for analysis of a broad range of chlorinated, brominated, and fluorinated POPs in human blood.

After establishing that the methods were effective, the two methods were applied to human plasma samples to examine the background levels of a broad range of POPs in human plasma samples among elderly men and women from Sweden and to assess the influence of gender. Levels of a wide range of chlorinated, brominated, and fluorinated compounds were determined in plasma samples collected during 2001-2004 from 1, 016 (50.2% women) 70 year-old participants from the population-based Prospective Study of the Vasculature in Uppsala Seniors (PIVUS). The POPs studied were 16 polychlorinated biphenyls (PCBs), 5 organochlorine pesticides (OCPs), 1 dioxin, 1 brominated flame retardant as well as 14 perfluoroalkyl substances (PFAS) including structural perfluorooctane sulfonic acid (PFOS) isomers. The majority of the studied compounds were detected in the 70-100% of the participants. The structural PFOS isomers were successfully quantified in a sub-sample of 25 men and women. Furthermore, gender differences in the concentrations of the POPs studied showed that the majority of chlorinated and brominated compounds were significantly different when comparing men and women in the study, while the concentrations of the fluorinated compounds were found to be less influenced by gender.

This thesis has, by using the developed high-throughput methods requiring only small amounts of human blood, provided background exposure information of a broad range of POPs for an epidemiological study.

Place, publisher, year, edition, pages
Örebro: Örebro universitet, 2013. p. 70
Series
Örebro Studies in Chemistry, ISSN 1651-4270 ; 12
Keywords
Human blood, POPs, Perfluoroalkylated substances, Structural isomers, Sample preparation, Column-switch, Mass spectrometry
National Category
Chemical Sciences
Research subject
Chemistry
Identifiers
urn:nbn:se:oru:diva-28456 (URN)978-91-7668-936-3 (ISBN)
Public defence
2013-05-31, Hörsal B, Billbergska huset, Örebro universitet, Fakultetsgatan 1, Örebro, 10:00 (English)
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Supervisors
Available from: 2013-03-22 Created: 2013-03-22 Last updated: 2017-10-17Bibliographically approved

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Salihovic, SamiraLindström, Gunillavan Bavel, Bert

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