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Exposure time-dependent effects on the relative potencies and additivity of PAHs in the Ah receptor-based H4IIE-luc bioassay
Örebro University, School of Science and Technology.
Örebro University, School of Science and Technology.
Örebro University, School of Science and Technology.ORCID iD: 0000-0001-7338-2079
2012 (English)In: Environmental Toxicology and Chemistry, ISSN 0730-7268, E-ISSN 1552-8618, Vol. 31, no 5, p. 1149-1157Article in journal (Refereed) Published
Abstract [en]

In the present study, relative potency factors (REPs) of 16 individual polycyclic aromatic hydrocarbons (PAHs) were investigated using the H4IIE-luc bioassay. Exposure time-dependent effects on the REPs were examined using 24, 48, and 72?h of exposure. Seven different mixtures of PAHs were tested for additivity at an exposure time of 24?h. Three of the PAH mixtures were also studied at 48 and 72?h of exposure. The mixture toxicities were predicted using the REP concept and the concentration addition (CA) model. Relative potency factor values investigated in the present study were similar to those reported in earlier studies. Declining REPs with an increasing exposure time were shown for all PAHs, indicating that this bioassay approach could be developed to assess the persistency of aryl hydrocarbon receptor (AhR) agonistic PAHs and in the risk assessment of complex PAH mixtures. The results from the mixture studies indicated that additive interactions of PAHs are time dependent. Generally, 48- and 72-h exposures resulted in biological effects that were similar to the CA and REP model predictions, while these models tended to underestimate the effect, to some extent, in the 24-h exposure, at least for the mixtures containing two to four PAHs. Thus, it cannot be ruled out that in the 24-h exposures, the tested PAH mixtures had slight synergistic effects. Further research is needed to identify and test additional AhR activating PAHs and investigate whether the effects in the H4IIE-luc bioassay are additive for more complex samples containing both PAHs and other AhR-activating contaminants. Also, the observed superinduction of luciferase by PAH-mixes warrants studies of whether this also can occur for relevant AhR-mediated endpoints in vivo. 

Place, publisher, year, edition, pages
Wiley-Blackwell, 2012. Vol. 31, no 5, p. 1149-1157
Keywords [en]
PAHs, AhR agonists, Relative potencies, Mixture toxicity, Concentration-addition
National Category
Chemical Sciences Biological Sciences
Research subject
Biology; Chemistry
Identifiers
URN: urn:nbn:se:oru:diva-22823DOI: 10.1002/etc.1776ISI: 000302909800032PubMedID: 22328298Scopus ID: 2-s2.0-84859877788OAI: oai:DiVA.org:oru-22823DiVA, id: diva2:526573
Available from: 2012-05-14 Created: 2012-05-14 Last updated: 2017-12-07Bibliographically approved
In thesis
1. Chemical and bioanalytical characterisation of PAH-contaminated soils: identification, availability and mixture toxicity of AhR agonists
Open this publication in new window or tab >>Chemical and bioanalytical characterisation of PAH-contaminated soils: identification, availability and mixture toxicity of AhR agonists
2013 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Contaminated soils are a worldwide problem. Polycyclic aromatic hydrocarbons (PAHs) are common contaminants in soil at former industrial areas, especially at old gasworks sites, gas stations and former wood impregnation facilities. Risk assessments of PAHs in contaminated soils are usually based on chemical analysis of a small number of individual PAHs, which only constitute a small part of the complex cocktail of hundreds of PAHs and other related polycyclic aromatic compounds (PACs) in the soils. Generally, the mixture composition of PAH-contaminated soils is rarely known and the mechanisms of toxicity and interactions between the pollutants are far from fully understood.

The main objective of this thesis was to characterize remediated PAHcontaminated soils by use of a chemical and bioanalytical approach. Bioassay specific relative potency (REP) values for 38 PAHs and related PACs were developed in the sensitive H4IIE-luc bioassay and used in massbalance analysis of remediated PAH contaminated soils, to assess the contribution of chemically quantified compounds to the overall aryl hydrocarbon receptor (AhR)-mediated activity observed in the H4IIE-luc bioassay. Mixtures studies showed additive AhR-mediated effects of PACs, including PAHs, oxy PAHs, methylated PAHs and azaarenes, in the bioassay, which supports the use of REP values in risk assessment. The results from the chemical and bioassay analysis showed that PAH-contaminated soils contained a large fraction of AhR activating compounds whose effect could not be explained by chemical analysis of the 16 priority PAHs. Further chemical identification and biological studies are necessary to determine whether these unknown substances pose a risk to human health or the environment. Results presented in this thesis are an important step in the development of AhR-based bioassay analysis and risk assessment of complex PAH-contaminated samples.

Place, publisher, year, edition, pages
Örebro: Örebro universitet, 2013. p. 60
Series
Örebro Studies in Chemistry, ISSN 1651-4270 ; 13
Keywords
Polycyclic aromatic compounds, Soil, Risk assessment, Mixture studies, AhR-mediated activity, REPs, GC/MS, H4IIE-luc bioassay
National Category
Chemical Sciences
Research subject
Chemistry
Identifiers
urn:nbn:se:oru:diva-30070 (URN)978-91-7668-961-5 (ISBN)
Public defence
2013-09-27, Hörsalen, Billbergska huset, Örebro universitet, Fakultetsgatan 1, 701 82 Örebro, 10:00 (Swedish)
Opponent
Supervisors
Funder
Swedish Environmental Protection AgencyKnowledge Foundation
Note

Other funders: Sparbanksstiftelsen Nya and Ångpanneföreningen

Available from: 2013-07-30 Created: 2013-07-30 Last updated: 2017-10-17Bibliographically approved

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Larsson, MariaEngwall, Magnus

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