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Oral immunosuppressive medication for growing pigs in transplantation studies
Department of Clinical Sciences, Section of Comparative Medicine, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Örebro University, School of Health and Medical Sciences, Örebro University, Sweden. (Department of Clinical Medicine)
Division of Transplantation Surgery, CLINTEC, Karolinska Institutet, Stockholm, Sweden.
2012 (English)In: Laboratory Animals. Journal of the Laboratory Animal Science Association, ISSN 0023-6772, E-ISSN 1758-1117, Vol. 46, no 2, p. 148-151Article in journal (Refereed) Published
Abstract [en]

Immunosuppressive (IS) medication is needed to avoid graft rejection in porcine transplantation models. An ideal IS therapy should have no side-effects, but increased susceptibility to infections, disturbed intestinal microflora and toxic effects on organs and tissues are commonly reported. The aim of the present study was to design an IS protocol with tacrolimus and mycophenolic acid to be used for maintenance therapy in the post-transplant period. An eligible whole blood trough value for tacrolimus was 5-15 mu g/L. Conventional specific pathogen-free pigs were fitted with an indwelling catheter under general anaesthesia, and after the acclimatization period three groups were formed: group A (n = 4) received 0.15 mg/kg body weight (BW) twice daily tacrolimus and 500 mg twice daily mycophenolic acid; group B (n = 4) received 0.3 mg/kg BW twice daily tacrolimus and 500 mg twice daily mycophenolic acid; group C (n = 2) did not receive any medication. Daily clinical examinations and analyses of blood concentrations of tacrolimus and glucose were performed. Total and differential white blood cell counts, enzyme activities, bilirubin and electrolyte concentrations were measured every fourth day. At the end of the experiment, the pigs were killed with an overdose of pentobarbital intravenously and a necropsy was performed immediately. All animals seemed to tolerate the IS treatment well. No alterations in their clinical state of health were observed throughout the study and daily weight gain was similar for the three groups. The necropsy did not reveal any pathological findings related to medication. The study showed that 0.25 mg/kg BW twice daily tacrolimus and 500 mg twice daily mycophenolic acid would be an appropriate maintenance dosage for conventional pigs.

Place, publisher, year, edition, pages
Thousand Oaks, USA: Sage Publications, 2012. Vol. 46, no 2, p. 148-151
Keywords [en]
Tacrolimus, mycophenolic acid, transplantation, maintenance dosage, swine
National Category
Medical and Health Sciences Clinical Laboratory Medicine
Research subject
Medicine
Identifiers
URN: urn:nbn:se:oru:diva-23699DOI: 10.1258/la.2012.011152ISI: 000304580600010PubMedID: 22398272Scopus ID: 2-s2.0-84864865498OAI: oai:DiVA.org:oru-23699DiVA, id: diva2:536804
Funder
Swedish Research Council FormasAvailable from: 2012-06-25 Created: 2012-06-25 Last updated: 2018-05-09Bibliographically approved

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Kruse, Robert

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