oru.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Role of NLRP3 and CARD8 in the regulation of TNF-α induced IL-1β release in vascular smooth muscle cells
Department of Clinical Medicine, School of Health and Medical Science, Örebro University, Örebro, Sweden.
Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.ORCID iD: 0000-0002-0278-4510
Show others and affiliations
2012 (English)In: International Journal of Molecular Medicine, ISSN 1107-3756, E-ISSN 1791-244X, Vol. 30, no 3, p. 697-702Article in journal (Refereed) Published
Abstract [en]

Interleukin (IL)-1β is known to be activated by the inflammasome. Inflammasome activities depend on a plethora of moieties including NLRP3 and CARD8, which have been reported to be associated with several inflammatory diseases. Aortic smooth muscle cells (AOSMCs) were transfected with siRNA targeting the NLRP3 and CARD8 genes, followed by tumor necrosis factor-α (TNF-α) treatment. We found that TNF-α induces IL-1β, IL-1Ra and NLRP3 genes but not CARD8. Silencing of the NLRP3 gene significantly decreased IL-1β expression and release, the IL-1Ra expression showed a borderline non-significant increment, while CARD8 knockdown did not affect the IL-1β and IL-1Ra mRNA expression or IL-1β protein release. Our results suggest that mainly NLRP3 plays a role in the regulation of IL-1β expression and release in AOSMC and could be a potential future target for the treatment of atherosclerosis and other inflammatory diseases.

Place, publisher, year, edition, pages
Athens, Greece: Spandidos Publications , 2012. Vol. 30, no 3, p. 697-702
Keywords [en]
Atherosclerosis, NLRP3, inflammasome, CARD8, vascular cells, inflammation
National Category
Immunology in the medical area
Research subject
Biomedicine; Medicine
Identifiers
URN: urn:nbn:se:oru:diva-23894DOI: 10.3892/ijmm.2012.1026ISI: 000307139400034PubMedID: 22711073Scopus ID: 2-s2.0-84865167444OAI: oai:DiVA.org:oru-23894DiVA, id: diva2:539657
Funder
Swedish Research CouncilSwedish Heart Lung Foundation
Note

Funding Agencies:

Swedish Fund for Research without Animal Experiments

Swedish Heart and Lung Association

Foundation of Olle Engkvist

Available from: 2012-07-04 Created: 2012-07-04 Last updated: 2018-01-12Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedScopus

Authority records BETA

Elmabsout, Ali AteiaBengtsson, TorbjörnSirsjö, AllanFransén, Karin

Search in DiVA

By author/editor
Elmabsout, Ali AteiaBengtsson, TorbjörnSirsjö, AllanFransén, Karin
By organisation
School of Health and Medical Sciences, Örebro University, Sweden
In the same journal
International Journal of Molecular Medicine
Immunology in the medical area

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 523 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf