oru.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Perfluorooctane sulfonate increases β-oxidation of palmitic acid in chicken liver
Örebro University, School of Science and Technology.
Örebro University, School of Science and Technology.
Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.ORCID iD: 0000-0001-8102-1804
Örebro University, School of Science and Technology.ORCID iD: 0000-0001-7338-2079
2012 (English)In: Environmental science and pollution research international, ISSN 0944-1344, E-ISSN 1614-7499, Vol. 19, no 5, p. 1859-1863Article in journal (Refereed) Published
Abstract [en]

Purpose: Perfluorooctane sulfonate (PFOS) belongs to a group of chemicals called perfluoroalkyl acids that have been extensively used in various applications such as stain and oil resistant treatments for fabrics, fire-fighting foams, and insecticides. These chemicals present an environmental and health risk being present in many samples both in wildlife and humans. In this study, we investigate the effect of PFOS on fatty acid β-oxidation in developing chicken embryos.

Methods: Fertilized chicken eggs were exposed in ovo to PFOS at day 4 of incubation. On day 10, the eggs were dissected and livers were incubated in vitro with (3)H-palmitic acid for 2 h. The media were collected, and after clean up, the amount of tritiated water was measured with liquid scintillation counting to determine the rate of palmitic acid β-oxidation.

Results: PFOS was found to induce fatty acid β-oxidation at doses starting from a lowest observed effect level (LOEL) of 0.1 μg/g egg weight. Maximum induction of 77 % compared to control was seen at 0.3 μg/g.

Conclusions: The administered doses in which effects are seen are around and even lower than the levels that can be found in wild populations of birds. General population human levels are a factor of two to three times lower than the LOEL value of this study. The environmental contamination of PFOS therefore presents a possibility of effects in wild populations of birds.

Place, publisher, year, edition, pages
Heidelberg, Germany: Springer Berlin/Heidelberg, 2012. Vol. 19, no 5, p. 1859-1863
Keywords [en]
Perfluorooctane sulfonate, PFOS, exposure, chicken, palmitic acid, β-oxidation, tritium
National Category
Medical and Health Sciences Biological Sciences Biomedical Laboratory Science/Technology
Research subject
Biology; Biomedical Laboratory Science
Identifiers
URN: urn:nbn:se:oru:diva-24853DOI: 10.1007/s11356-012-0869-1ISI: 000305884600053PubMedID: 22441698Scopus ID: 2-s2.0-84863225035OAI: oai:DiVA.org:oru-24853DiVA, id: diva2:546453
Funder
Swedish Research Council FormasAvailable from: 2012-08-23 Created: 2012-08-23 Last updated: 2017-12-07Bibliographically approved
In thesis
1. Comparative avian developmental toxicity of PFAAs
Open this publication in new window or tab >>Comparative avian developmental toxicity of PFAAs
2013 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Perfluoroalkyl acids (PFAAs) are persistent organic pollutants that can commonly be found in environmental matrixes and wildlife from all over the globe. The PFAAs have been used in applications such as water and dirt repelling treatments for textiles, oil-resistant paper coatings and fire-fighting foams. Four studies were designed to evaluate the occurrence of PFAAs in Swedish populations of birds, the developmental toxicity of different PFAAs and species sensitivity differences as well as possible modes of action for the toxicity. The studied species were domestic chicken, and the wild species great cormorant and herring gull. Cormorant and gull eggs were collected from bird colonies in Lake Vänern, Sweden. Chemical analyses were performed on some of the eggs to determine the occurrence of 15 PFAAs in the eggs. The other eggs and eggs of domestic chicken were incubated and injected with solutions of the PFAAs PFOS, PFOA, PFBS and PFUnDA. The eggs were candled every 1-3 days to determine viability. High levels of PFAAs, mainly PFOS followed by PFUnDA, were found in the herring gull and great cormorant eggs. PFOS was found at concentrations up to 1163 ng/g and 771 ng/g in cormorant and herring gull, respectively. In the toxicity tests, chicken was found to be more sensitive than the wild species and cormorant was in general the least sensitive species. PFOA was found to be the most toxic of the chemicals followed by PFOS, PFBS and PFUnDA in decreasing order. Comparing these results with the levels of these chemicals found in the eggs of herring gull and great cormorant, PFOS is the chemical of most concern. Although PFOA had the highest toxicity, the levels found in the eggs were very low. In an additional study, the hepatic β-oxidation in developing chicken embryos after in ovo exposure to PFOS was studied with a tritium release assay. PFOS was found to increase the β-oxidation of palmitic acid at PFOS concentrations 3-7 times lower than the average egg levels in cormorant and herring gull. Therefore the occurrence of effects on the fatty acid metabolism cannot be ruled out. The doses of effect on embryo survival in the toxicity and the levels found in the herring gulls and cormorants gives a small margin of safety for the wild populations. Continued environmental monitoring and further studies on the toxicity of PFAAs that occur at high environmental concentrations is important.

Place, publisher, year, edition, pages
Örebro: Örebro universitet, 2013. p. 54
Series
Örebro Studies in Biology, ISSN 1650-8793 ; 7
Keywords
chicken, herring gull, cormorant, PFAA, PFOS, PFOA, toxicity, levels
National Category
Biological Sciences Environmental Sciences
Research subject
Biology
Identifiers
urn:nbn:se:oru:diva-30099 (URN)978-91-7668-959-2 (ISBN)
Public defence
2013-10-04, Hörsalen, Billbergska huset, Örebro universitet, Fakultetsgatan 1, Örebro, 13:00 (Swedish)
Opponent
Supervisors
Available from: 2013-08-01 Created: 2013-08-01 Last updated: 2017-10-17Bibliographically approved
2. Development of bioassays for risk assessment of PAHs and PFASs in the environment
Open this publication in new window or tab >>Development of bioassays for risk assessment of PAHs and PFASs in the environment
2013 (English)Doctoral thesis, comprehensive summary (Other academic)
Place, publisher, year, edition, pages
Örebro: Örebro universitet, 2013. p. 72
Series
Örebro Studies in Biology, ISSN 1650-8793 ; 8
National Category
Environmental Sciences Biological Sciences
Research subject
Biology
Identifiers
urn:nbn:se:oru:diva-30068 (URN)978-91-7668-965-3 (ISBN)
Public defence
2013-10-11, Hörsalen, Billbergska huset, Örebro universitet, Fakultetsgatan 1, Örebro, 10:00 (English)
Opponent
Supervisors
Available from: 2013-07-30 Created: 2013-07-30 Last updated: 2017-10-17Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedScopus

Authority records BETA

Nordén, MarcusWestman, OlaVenizelos, NikolaosEngwall, Magnus

Search in DiVA

By author/editor
Nordén, MarcusWestman, OlaVenizelos, NikolaosEngwall, Magnus
By organisation
School of Science and TechnologySchool of Health and Medical Sciences, Örebro University, Sweden
In the same journal
Environmental science and pollution research international
Medical and Health SciencesBiological SciencesBiomedical Laboratory Science/Technology

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 488 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf