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Altered T-cell responses by the periodontal pathogen Porphyromonas gingivalis
Örebro University, School of Health and Medical Sciences, Örebro University, Sweden. Division of Clinical Medicine,, Faculty of Health and Medical Sciences, Örebro University, Örebro, Sweden.
Örebro University, School of Medicine, Örebro University, Sweden. Division of Clinical Medicine, Faculty of Health and Medical Sciences, Örebro University, Örebro, Sweden.
2012 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 7, no 9, article id e45192Article in journal (Refereed) Published
Abstract [en]

Several studies support an association between the chronic inflammatory diseases periodontitis and atherosclerosis with a crucial role for the periodontal pathogen Porphyromonas gingivalis. However, the interplay between this pathogen and the adaptive immune system, including T-cells, is sparsely investigated. Here we used Jurkat T-cells to determine the effects of P. gingivalis on T-cell-mediated adaptive immune responses. We show that viable P. gingivalis targets IL-2 expression at the protein level. Initial cellular events, including ROS production and [Ca2+]i, were elevated in response to P. gingivalis, but AP-1 and NF-κB activity dropped below basal levels and T-cells were unable to sustain stable IL-2 accumulation. IL-2 was partially restored by Leupeptin, but not by Cathepsin B Inhibitor, indicating an involvement of Rgp proteinases in the suppression of IL-2 accumulation. This was further confirmed by purified Rgp that caused a dose-dependent decrease in IL-2 levels. These results provide new insights of how this periodontal pathogen evades the host adaptive immune system by inhibiting IL-2 accumulation and thus attenuating T-cell proliferation and cellular communication.

Place, publisher, year, edition, pages
San Francisco, USA: Public Library Science , 2012. Vol. 7, no 9, article id e45192
National Category
Medical and Health Sciences Immunology Biochemistry and Molecular Biology
Research subject
Biomedicine; Medicine
Identifiers
URN: urn:nbn:se:oru:diva-25336DOI: 10.1371/journal.pone.0045192ISI: 000308738500126PubMedID: 22984628Scopus ID: 2-s2.0-84866342642OAI: oai:DiVA.org:oru-25336DiVA, id: diva2:547056
Funder
Swedish Research CouncilSwedish Heart Lung FoundationAvailable from: 2012-08-27 Created: 2012-08-27 Last updated: 2018-08-27Bibliographically approved

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Khalaf, HazemBengtsson, Torbjörn

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