oru.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Sequencing of a tick-borne encephalitis virus from Ixodes ricinus reveals a thermosensitive RNA switch significant for virus propagation in ectothermic arthropods
School of Life Sciences, Södertörn University, Huddinge, Sweden.
School of Life Sciences, Södertörn University, Huddinge, Sweden; Department of Genetics, Microbiology and Toxicology, Stockholm University, Stockholm, Sweden.
School of Life Sciences, Södertörn University, Huddinge, Sweden.
School of Life Sciences, Södertörn University, Huddinge, Sweden.
Show others and affiliations
2011 (English)In: Vector Borne and Zoonotic Diseases, ISSN 1530-3667, E-ISSN 1557-7759, Vol. 11, no 6, p. 649-658Article in journal (Refereed) Published
Abstract [en]

Tick-borne encephalitis virus (TBEV) is a flavivirus with major impact on global health. The geographical TBEV distribution is expanding, thus making it pivotal to further characterize the natural virus populations. In this study, we completed the earlier partial sequencing of a TBEV pulled out of a pool of RNA extracted from 115 ticks collected on Torö in the Stockholm archipelago. The total RNA was sufficient for all sequencing of a TBEV genome (Torö-2003), without conventional enrichment procedures such as cell culturing or suckling mice amplification. To our knowledge, this is the first time that the genome of TBEV has been sequenced directly from an arthropod reservoir. The Torö-2003 sequence has been characterized and compared with other TBE viruses. In silico analyses of secondary RNA structures formed by the two untranslated regions revealed a temperature-sensitive structural shift between a closed replicative form and an open AUG accessible form, analogous to a recently described bacterial thermoswitch. Additionally, novel phylogenetic conserved structures were identified in the variable part of the 3'-untranslated region, and their sequence and structure similarity when compared with earlier identified structures suggests an enhancing function on virus replication and translation. We propose that the thermo-switch mechanism may explain the low TBEV prevalence often observed in environmentally sampled ticks. Finally, we were able to detect variations that help in the understanding of virus adaptations to varied environmental temperatures and mammalian hosts through a comparative approach that compares RNA folding dynamics between strains with different mammalian cell passage histories.

Place, publisher, year, edition, pages
Mary Ann Liebert, 2011. Vol. 11, no 6, p. 649-658
Keywords [en]
conserved secondary RNA structures in the 3 '-UTR; genome of Swedish TBEV; riboswitch; RNA thermosensor
National Category
Infectious Medicine
Identifiers
URN: urn:nbn:se:oru:diva-27102DOI: 10.1089/vbz.2010.0105ISI: 000291717500010PubMedID: 21254926Scopus ID: 2-s2.0-79959411565OAI: oai:DiVA.org:oru-27102DiVA, id: diva2:601455
Funder
The Foundation for Baltic and East European StudiesAvailable from: 2013-01-29 Created: 2013-01-29 Last updated: 2019-04-04Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedScopus

Authority records BETA

Melik, WessamJohansson, Magnus

Search in DiVA

By author/editor
Melik, WessamJohansson, Magnus
In the same journal
Vector Borne and Zoonotic Diseases
Infectious Medicine

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 431 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf