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Identification and sequence of a nifJ-like gene in Rhodospirillum rubrum: partial characterization of a mutant unaffected in nitrogen fixation
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1996 (English)In: Molecular Microbiology, ISSN 0950-382X, E-ISSN 1365-2958, Vol. 20, no 3, p. 559-568Article in journal (Refereed) Published
Abstract [en]

A nifJ-like gene was identified in the photosynthetic purple non-sulphur bacterium Rhodospirillum rubrum. A DNA segment hybridizing to Klebsiella pneumoniae nifJ was isolated, the gene was inactivated, and a mutant strain, SNJ-1, was constructed by allele replacement. The mutation was confirmed by DNA sequencing. Northern blotting and by the lack of pyruvate oxidoreductase activity. This is the first report of a nifJ-like gene in photosynthetic bacteria. Unexpectedly, SNJ-1 was capable of nitrogen fixation, and growth was similar to the wild-type strain under all conditions investigated. Therefore, this is also the first demonstration that a nifJ homologue, when present, is not essential for nitrogen fixation in a diazotroph. The nifJ-like gene was sequenced and found to have considerable similarity to published nifJ gene sequences from other organisms. By primer extension, the initiation site for transcription was located, and a typical sigma 54 promoter sequence was identified.

Place, publisher, year, edition, pages
1996. Vol. 20, no 3, p. 559-568
National Category
Biochemistry and Molecular Biology
Research subject
Medicine
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URN: urn:nbn:se:oru:diva-27114PubMedID: 8736535OAI: oai:DiVA.org:oru-27114DiVA, id: diva2:601482
Available from: 2013-01-29 Created: 2013-01-29 Last updated: 2017-12-06Bibliographically approved

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Johansson, Magnus

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