oru.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
High-throughput live cell imaging reveals differential inhibition of tumor cell proliferation by human fibroblasts
Department of Microbiology, Tumor and Cell Biology (MTC), Center for Integrative Recognition in the Immune System (IRIS), Karolinska Institutet, Stockholm, Sweden; Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institute, Stockholm, Sweden.
Department of Microbiology, Tumor and Cell Biology (MTC), Center for Integrative Recognition in the Immune System (IRIS), Karolinska Institutet, Stockholm, Sweden.
Department of Microbiology, Tumor and Cell Biology (MTC), Center for Integrative Recognition in the Immune System (IRIS), Karolinska Institutet, Stockholm, Sweden.
Department of Microbiology, Tumor and Cell Biology (MTC), Center for Integrative Recognition in the Immune System (IRIS), Karolinska Institutet, Stockholm, Sweden.
Show others and affiliations
2011 (English)In: International Journal of Cancer, ISSN 0020-7136, E-ISSN 1097-0215, Vol. 128, no 12, p. 2793-2802Article in journal (Refereed) Published
Abstract [en]

Increasing evidence indicates that cancer development requires changes both in the precancerous cells and in their microenvironment. To study one aspect of the microenvironmental control, we departed from Michael Stoker's observation (Stroker et al, J Cell Sci 1966;1:297-310) that normal fibroblasts can inhibit the growth of admixed cancer cells (neighbour suppression). We have developed a high-throughput microscopy and image analysis system permitting the examination of live mixed cell cultures growing on 384-well plates, at the single cell level and over time. We have tested the effect of 107 samples of low passage number (<5) primary human fibroblasts from pediatric and adult donors, on the growth of six human tumor cell lines. Three of the lines were derived from prostate carcinomas, two from lung carcinomas and one was an EBV transformed lymphoblastoid line. Labeled tumor cells were grown in the presence of unlabeled fibroblasts. The majority of the tested fibroblasts inhibited the proliferation of the tumor cells, compared to the control cultures where labeled tumor cells were co-cultured with unlabeled tumor cells. The proliferation inhibiting effect of the fibroblasts differed depending on their site of origin and the age of the donor. Inhibition required direct cell contact. Mouse 3T3 fibroblasts inhibited the growth of SV40-transformed 3T3 cells and human tumor cells, showing that the inhibitory effect could prevail across the species barrier. Our high-throughput system allows the quantitative analysis of the inhibitory effect of fibroblasts on the population level and the exploration of differences depending on the source of the normal cells.

Place, publisher, year, edition, pages
Wiley-Blackwell, 2011. Vol. 128, no 12, p. 2793-2802
Keywords [en]
neighbour suppression; fibroblasts; tumor inhibition; high throughput microscopy
National Category
Cancer and Oncology
Identifiers
URN: urn:nbn:se:oru:diva-27427DOI: 10.1002/ijc.25612ISI: 000289986900004PubMedID: 20715102Scopus ID: 2-s2.0-79955461174OAI: oai:DiVA.org:oru-27427DiVA, id: diva2:603683
Funder
Swedish Cancer SocietySwedish Research Council
Note

Funding Agencies:

Karolinska Institutet

Nyckelfonden Örebro  

Cancer Research Institute (CRI), NY, USA  

Concern Foundation, LA, USA 

Available from: 2013-02-06 Created: 2013-02-06 Last updated: 2018-05-04Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedScopus

Authority records BETA

Andrén, OveJohansson, J-EAndersson, Swen-Olof

Search in DiVA

By author/editor
Andrén, OveJohansson, J-EAndersson, Swen-Olof
By organisation
School of Health and Medical Sciences
In the same journal
International Journal of Cancer
Cancer and Oncology

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 71 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf