oru.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Long-term molecular epidemiology of Staphylococcus epidermidis blood culture isolates from patients with haematological malignancies
Örebro University, School of Health and Medical Sciences, Örebro University, Sweden. Department of Medicine, Division of Haematology, Örebro University Hospital, SE-701 85 Örebro, SWEDEN.
Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, SE-701 85 Örebro, Sweden.
Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, SE-701 85 Örebro, Sweden.
Örebro University, School of Health and Medical Sciences, Örebro University, Sweden. Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, SE-701 85 Örebro, Sweden.ORCID iD: 0000-0001-5939-2932
(English)Manuscript (preprint) (Other academic)
Abstract [en]

Staphylococcus epidermidis is an important cause of bloodstream infections in patients with haematological malignancies. Knowledge of the long-term epidemiology of these infections is limited. We surveyed all S. epidermidis blood culture isolates from patients treated for haematological malignancies at the University Hospital of Örebro, Sweden from 1980 to 2009. A total of 373 S. epidermidis isolates were identified and multilocus sequence typing and standard antibiotic susceptibility testing were employed to characterize these isolates. The majority of the isolates 361/373 (97%) belonged to clonal complex 2, and the 373 isolates were divided into 45 sequence types (STs); Simpson’s Diversity Index was 0.56. The most prevalent STs were ST2 (243/373, 65%) and ST215 (28/373, 8%). These two STs were isolated during the entire study period, and together caused temporal peaks in the incidence of positive blood cultures of S. epidermidis. Methicillin resistance was detected in 213/273 (78%) of all isolates. In the two predominating STs, ST2 and ST215, the proportion of methicillin resistance was 257/271 (95%); 234/271 (86%) displayed a multidrug-resistant phenotype. In conclusion, in this long-term study of patients with haematological malignancies, we demonstrate a predominance of methicillin-resistant ST2 among S. epidermidis blood culture isolates.

Keywords [en]
Staphylococcus epidermidis, Bloodstream infection, Haematological malignancy, Multilocus sequence typing, Coagulase-negative staphylococci, Molecular epidemiology, Healthcare-associated infection
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:oru:diva-32423OAI: oai:DiVA.org:oru-32423DiVA, id: diva2:664635
Available from: 2013-11-15 Created: 2013-11-15 Last updated: 2017-10-17Bibliographically approved
In thesis
1. Coagulase-negative staphylococci in hematological malignancy
Open this publication in new window or tab >>Coagulase-negative staphylococci in hematological malignancy
2013 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Bacterial infections are common in hematological malignancy. Coagulase-negative staphylococci (CoNS) are among the most prevalent causes of bacteremia in patients with hematological malignancies.

In this thesis, different aspects of CoNS in hematological malignancy have been studied in four papers:

In paper 1, CoNS blood culture isolates from patients with hematological malignancies treated at the University Hospital of Örebro from 1980 to 2009 were revaluated for the presence of reduced sensitivity to glycopeptides. A high incidence of heterogeneous-intermediate glycopeptide resistance was observed and there was a trend towards increasing incidence of this phenotype over time.

In paper 2, the colonization pattern of CoNS among patients undergoing intensive chemotherapy for hematological malignancy was investigated. A successive homogenization and an accumulation of CoNS phenotypes mutually present in a majority of included patients were demonstrated.

In paper 3, a PCR method to determine the clinical significance of positive blood cultures of the CoNS species Staphylococcus epidermidis was evaluated. The test failed to discriminate bloodstream infection from blood culture contamination.

Finally, in paper 4, the long-term molecular epidemiology of S. epidermidis blood culture isolates from patients with hematological malignancies was studied with multilocus sequence typing. A predominance of sequence type 2 was demonstrated during the entire 30 year study period.

In conclusion, the results are consistent with that CoNS have established as important pathogens by its capacity to colonize the human skin, its ability to reside and spread in the hospital environment and its rapid adaptation to stressors such as antimicrobials.

Place, publisher, year, edition, pages
Örebro: Örebro universitet, 2013. p. 56
Series
Örebro Studies in Medicine, ISSN 1652-4063 ; 95
Keywords
coagulase-negative staphylococci, hematological malignancy, Staphylococcus epidermidis, healthcare-associated infection, antibiotic susceptibility, molecular epidemiology, bloddstream infection, bacteremia
National Category
Hematology
Research subject
Medicine
Identifiers
urn:nbn:se:oru:diva-30750 (URN)978-91-7668-970-7 (ISBN)
Public defence
2013-10-15, Wilandersalen, Universitetssjukhuset i Örebro, S. Grev Roseng. 18, 703 62 ÖREBRO, 09:00 (Swedish)
Opponent
Supervisors
Available from: 2013-09-09 Created: 2013-09-09 Last updated: 2017-10-17Bibliographically approved

Open Access in DiVA

No full text in DiVA

Authority records BETA

Ahlstrand, ErikSöderquist, Bo

Search in DiVA

By author/editor
Ahlstrand, ErikSöderquist, Bo
By organisation
School of Health and Medical Sciences, Örebro University, Sweden
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar

urn-nbn

Altmetric score

urn-nbn
Total: 342 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf