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Time-dependent relative potency factors (REPS) for polycyclic aromatic hydrocarbons and their derivatives in the h4iie-luc
Örebro University, School of Science and Technology. (Man-Technology-Environment Research Centre (MTM))
Örebro University, School of Science and Technology. (Man-Technology-Environment Research Centre (MTM))ORCID iD: 0000-0003-4128-8226
Department of Biology & Chemistry and State Key Laboratory in Marine Pollution & School of Biological Sciences, University of Hong Kong, China; State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University,China.
Örebro University, School of Science and Technology. (Man-Technology-Environment Research Centre (MTM))ORCID iD: 0000-0001-7338-2079
(English)Manuscript (preprint) (Other academic)
Abstract [en]

The H4IIE-luc transactivation bioassay for aryl hydrocarbon receptor (AhR) agonists was used to investigate relative potency factors (REPs) of 22 individual polycyclic aromatic hydrocarbons (PAHs) and their oxygenated-, methylated- and N-containing derivatives (azaarenes), which are often present in PAH-contaminated soils. Naphthacene and dibenz[ah]acridine exhibited greater AhRmediated potency, whereas lesser-molecular azaarenes were less potent AhR agonists. Six oxy-PAHs had calculable Relative potencies (REPs), but their potencies were less than their parent PAHs. Unlike the parent, unsubstituted PAHs, oxidation of methylated PAHs seemed to increase the AhR-mediated potency of the compounds, with methylanthracene-9,10-dione being almost two times more potent than methylanthracene. Both bioassay and gas chromatography–mass spectrometry (GC/MS) analysis were used to examine the exposure time dependent effects on the REPs at 24, 48 and 72 h of exposure in the H4IIE-luc transactivation bioassay. Changes in concentrations of five compounds including the model reference 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in the cell culture wells were measured, and the amounts in the cell medium, the cells and adsorbed to the wells determined and the influence on the REPs was studied. Declining REP values with increased duration of exposure were shown for all compounds and proved to be a consequence of the metabolism of PAHs and PAH derivatives in H4IIe-luc cells. The present study provides new knowledge regarding the degradation and distribution of compounds in the wells during exposure.

Keywords [en]
Polycyclic aromatic compounds, monitoring, soils, organic contaminants, in vitro toxicology, toxic equivalents
National Category
Chemical Sciences
Research subject
Chemistry
Identifiers
URN: urn:nbn:se:oru:diva-32665OAI: oai:DiVA.org:oru-32665DiVA, id: diva2:676693
Available from: 2013-12-06 Created: 2013-12-06 Last updated: 2017-10-17Bibliographically approved
In thesis
1. Chemical and bioanalytical characterisation of PAH-contaminated soils: identification, availability and mixture toxicity of AhR agonists
Open this publication in new window or tab >>Chemical and bioanalytical characterisation of PAH-contaminated soils: identification, availability and mixture toxicity of AhR agonists
2013 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Contaminated soils are a worldwide problem. Polycyclic aromatic hydrocarbons (PAHs) are common contaminants in soil at former industrial areas, especially at old gasworks sites, gas stations and former wood impregnation facilities. Risk assessments of PAHs in contaminated soils are usually based on chemical analysis of a small number of individual PAHs, which only constitute a small part of the complex cocktail of hundreds of PAHs and other related polycyclic aromatic compounds (PACs) in the soils. Generally, the mixture composition of PAH-contaminated soils is rarely known and the mechanisms of toxicity and interactions between the pollutants are far from fully understood.

The main objective of this thesis was to characterize remediated PAHcontaminated soils by use of a chemical and bioanalytical approach. Bioassay specific relative potency (REP) values for 38 PAHs and related PACs were developed in the sensitive H4IIE-luc bioassay and used in massbalance analysis of remediated PAH contaminated soils, to assess the contribution of chemically quantified compounds to the overall aryl hydrocarbon receptor (AhR)-mediated activity observed in the H4IIE-luc bioassay. Mixtures studies showed additive AhR-mediated effects of PACs, including PAHs, oxy PAHs, methylated PAHs and azaarenes, in the bioassay, which supports the use of REP values in risk assessment. The results from the chemical and bioassay analysis showed that PAH-contaminated soils contained a large fraction of AhR activating compounds whose effect could not be explained by chemical analysis of the 16 priority PAHs. Further chemical identification and biological studies are necessary to determine whether these unknown substances pose a risk to human health or the environment. Results presented in this thesis are an important step in the development of AhR-based bioassay analysis and risk assessment of complex PAH-contaminated samples.

Place, publisher, year, edition, pages
Örebro: Örebro universitet, 2013. p. 60
Series
Örebro Studies in Chemistry, ISSN 1651-4270 ; 13
Keywords
Polycyclic aromatic compounds, Soil, Risk assessment, Mixture studies, AhR-mediated activity, REPs, GC/MS, H4IIE-luc bioassay
National Category
Chemical Sciences
Research subject
Chemistry
Identifiers
urn:nbn:se:oru:diva-30070 (URN)978-91-7668-961-5 (ISBN)
Public defence
2013-09-27, Hörsalen, Billbergska huset, Örebro universitet, Fakultetsgatan 1, 701 82 Örebro, 10:00 (Swedish)
Opponent
Supervisors
Funder
Swedish Environmental Protection AgencyKnowledge Foundation
Note

Other funders: Sparbanksstiftelsen Nya and Ångpanneföreningen

Available from: 2013-07-30 Created: 2013-07-30 Last updated: 2017-10-17Bibliographically approved

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Larsson, MariaHagberg, JessikaEngwall, Magnus

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