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Concentration-addition in risk assessment: prediction of potential AhR-mediated activity in multiple polycyclic aromatic compound (PAC) mixtures
Örebro University, School of Science and Technology. (Man-Technology-Environment Research Centre (MTM))
Department of Veterinary Biomedical Sciences & Toxicological Center, University of Saskatchewan, Saskatoon, Canada; Department of Zoology, and Center for Integrative Toxicology, Michigan State University, USA Department of Biology & Chemistry and State Key Laboratory in Marine Pollution, City University of Hong Kong, China; State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, China.
Örebro University, School of Science and Technology. (aMan-Technology-Environment Research Centre (MTM))ORCID iD: 0000-0001-7338-2079
(English)Manuscript (preprint) (Other academic)
Abstract [en]

Risk assessments of polycyclic aromatic hydrocarbons (PAHs) are complicated because these compounds exist in the environment as complex mixtures of hundreds of individual PAHs and other related polycyclic aromatic compounds (PACs). In this study, the hypothesis that concentration addition (CA) can be used to predict the aryl hydrocarbon receptor (AhR) mediated potency of PACs in mixtures containing various combinations of PACs. Effects of 18 mixtures composed of two to 23 PACs, which included PAHs, azaarenes and oxygenated PAHs, were examined by use of the AhR based H4IIE-luc bioassay. Since greater AhR-mediated activities have been observed in soils contaminated by PAH, investigations were done to test whether soil extract matrix or presence of noneffect PACs might affect responses of the H4IIE-luc bioassay. AhR-mediated activity of the mixture of PACs could be predicted by use of concentration addition. Additive behavior of PACs in multi component mixtures supported the hypothesis that use of concentration addition could be used in risk assessment of PAC- mixtures. However, independent action (IA) could not be used to predict the activity of mixtures of PACs. 

Keywords [en]
Polycyclic aromatic hydrocarbons, Independent action, concentration addition, H4IIE-luc
National Category
Chemical Sciences
Research subject
Chemistry
Identifiers
URN: urn:nbn:se:oru:diva-32667OAI: oai:DiVA.org:oru-32667DiVA, id: diva2:676705
Available from: 2013-12-06 Created: 2013-12-06 Last updated: 2017-10-17Bibliographically approved
In thesis
1. Chemical and bioanalytical characterisation of PAH-contaminated soils: identification, availability and mixture toxicity of AhR agonists
Open this publication in new window or tab >>Chemical and bioanalytical characterisation of PAH-contaminated soils: identification, availability and mixture toxicity of AhR agonists
2013 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Contaminated soils are a worldwide problem. Polycyclic aromatic hydrocarbons (PAHs) are common contaminants in soil at former industrial areas, especially at old gasworks sites, gas stations and former wood impregnation facilities. Risk assessments of PAHs in contaminated soils are usually based on chemical analysis of a small number of individual PAHs, which only constitute a small part of the complex cocktail of hundreds of PAHs and other related polycyclic aromatic compounds (PACs) in the soils. Generally, the mixture composition of PAH-contaminated soils is rarely known and the mechanisms of toxicity and interactions between the pollutants are far from fully understood.

The main objective of this thesis was to characterize remediated PAHcontaminated soils by use of a chemical and bioanalytical approach. Bioassay specific relative potency (REP) values for 38 PAHs and related PACs were developed in the sensitive H4IIE-luc bioassay and used in massbalance analysis of remediated PAH contaminated soils, to assess the contribution of chemically quantified compounds to the overall aryl hydrocarbon receptor (AhR)-mediated activity observed in the H4IIE-luc bioassay. Mixtures studies showed additive AhR-mediated effects of PACs, including PAHs, oxy PAHs, methylated PAHs and azaarenes, in the bioassay, which supports the use of REP values in risk assessment. The results from the chemical and bioassay analysis showed that PAH-contaminated soils contained a large fraction of AhR activating compounds whose effect could not be explained by chemical analysis of the 16 priority PAHs. Further chemical identification and biological studies are necessary to determine whether these unknown substances pose a risk to human health or the environment. Results presented in this thesis are an important step in the development of AhR-based bioassay analysis and risk assessment of complex PAH-contaminated samples.

Place, publisher, year, edition, pages
Örebro: Örebro universitet, 2013. p. 60
Series
Örebro Studies in Chemistry, ISSN 1651-4270 ; 13
Keywords
Polycyclic aromatic compounds, Soil, Risk assessment, Mixture studies, AhR-mediated activity, REPs, GC/MS, H4IIE-luc bioassay
National Category
Chemical Sciences
Research subject
Chemistry
Identifiers
urn:nbn:se:oru:diva-30070 (URN)978-91-7668-961-5 (ISBN)
Public defence
2013-09-27, Hörsalen, Billbergska huset, Örebro universitet, Fakultetsgatan 1, 701 82 Örebro, 10:00 (Swedish)
Opponent
Supervisors
Funder
Swedish Environmental Protection AgencyKnowledge Foundation
Note

Other funders: Sparbanksstiftelsen Nya and Ångpanneföreningen

Available from: 2013-07-30 Created: 2013-07-30 Last updated: 2017-10-17Bibliographically approved

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Larsson, MariaEngwall, Magnus

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