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Effect of oxidative stress on tryptophan and tyrosine uptake in cultured human fibroblasts: the  role of α tocotreinol: Oxidative stress and amino acid uptake
Örebro University, School of Science and Technology. (Neuropsychiatric research group, Nikolaos Venizelos)
2012 (English)Independent thesis Advanced level (degree of Master (Two Years)), 30 credits / 45 HE creditsStudent thesis
Abstract [en]

Aberrant transport of tryptophan and tyrosine is implicated in abnormal dopaminergic and serotoninergic transmissions in psychiatric disorders. Disturbed transport of tyrosine is a repeated finding in cultured fibroblasts from psychiatric patients. The causes for the aberrant transport are not clearly defined yet, but a general membrane dysfunction and altered amino acid transport in psychiatric patients are suggested as major factors. Cellular macromolecules (for example, membrane proteins and lipids) damage associated with oxidative stress are common phenomena in psychiatric patients and antioxidants are proven to be effective in the management of certain psychiatric disorders as therapeutic adjutants. Moreover, oxidative stress has been reported to inhibit the uptake of amino acids in vitro that can be reversed by the application of antioxidants. The aims of the present study were to assess the effect of hydrogen peroxide (H2O2) on tryptophan and tyrosine uptake, and to further investigate the role of α-tocotrienol in counteracting the effect of H2O2 on tryptophan and tyrosine uptake in cultured fibroblasts. Fibroblasts from a healthy control were treated with H2O2, α-tocotrienol and H2O2 and α-tocotrienol together, and the uptake of L-[14C] tyrosine and L-[3H] tryptophan was determined by using the cluster tray technique. Treatment of fibroblasts with H2O2 significantly (P<0.05) inhibited tyrosine uptake (15.4% inhibition), and the inhibition was prevented by the application of α-tocotrienol. The same treatment conditions did not affect the uptake of tryptophan at the specific tryptophan concentration used for the uptake assay in this study. The results of this study suggest that oxidative stress could be a cause for the aberrant tyrosine transport in fibroblasts from different psychiatric patients and provide a potential mechanism of action for antioxidants used in the treatment of psychiatric disorders; further studies are required to understand the role of oxidative stress on amino acid transporters.

Place, publisher, year, edition, pages
2012. , p. 21
Keywords [en]
Hydrogen peroxide, antioxidants, amino acid transport
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:oru:diva-33261OAI: oai:DiVA.org:oru-33261DiVA, id: diva2:690391
Subject / course
Biology
Presentation
2012-08-30, Orebro, 13:18 (English)
Supervisors
Examiners
Available from: 2014-04-16 Created: 2014-01-23 Last updated: 2017-10-17Bibliographically approved

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CiteExportLink to record
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