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Comparative study and characterization of mutagenicity and AhR-agonistic potency of contaminated soil, remediated soil, urban city soil and rural soil
Örebro University, School of Science and Technology. (MTM)
Örebro University, School of Science and Technology. (MTM)
2RWTH Aachen University, Inst. For Environmental Research, Department of Ecosystem Analysis, Aachen, Germany.
Örebro University, School of Science and Technology. (MTM)ORCID iD: 0000-0001-7338-2079
(English)Manuscript (preprint) (Other academic)
Abstract [en]

Current risk assessment of PAH-contaminated soils is usually based on chemical analysis of a small number of PAHs. The use of effect-based methods for risk assessment would yield results covering more of the effect of all the chemicals in the soil. To put such effect based data into context we tested a relative approach in which effects of contaminated soil were compared to clean rural and urban soils. This concept of relative risk assessment was tested by studying the mutagenic and AhR-agonistic potency of contaminated soil and urban soil compared to farm soil from selected ecological farms. A set of 21 soil samples was collected: 11 PAH-contaminated samples (collected in collaboration with three Swedish remediation companies), 5 urban samples (collected in Swedish cities) and 5 soil samples from ecological farms. The urban and rural samples were collected at the surface (0-10 cm deep), the contaminated samples were collected from piles during remediation (100-200 cm deep). To evaluate the toxicants in the soil sample, lipophilic sample extracts were tested in two different assays; (i) the Ames Fluctuation Assay (AFA) mutant strains TA98 and TA100 of Salmonella typhimurium with and without a metabolic activation system (rat-liver homogenate S9) to determine the mutagenic potential of the soil samples and (ii) the cell mechanism-specific H4IIE-luc assay to determine the Ahreceptor (AhR) activating potency of the soil extracts. The results showed clear mutagenicity, both direct and indirect, in one of the PAH-contaminated samples and three other PAH samples also demonstrated some mutagenic activity. The extracts from urban city soil showed mutagenicity in three of the 5 samples, while none of the ecological farm samples had mutagenic extracts. The bio-TEQ values were very high for all remediated samples and elevated in one urban sample. Bio-TEQ values were low in the ecological farm extracts. These findings demonstrate that the present investigation scheme using two different bioassays to determine the mutagenic potential and the Ah receptor activating potency of soil extracts is a suitable method for testing toxic properties of soil extracts. The concept of relative risk assessment using background samples from rural and urban areas and effect based testing shows promise for further development.

Keywords [en]
contaminated soil; genotoxicity; AhR-inducing; bioassay, toxicity
National Category
Biological Sciences
Research subject
Biology
Identifiers
URN: urn:nbn:se:oru:diva-33947OAI: oai:DiVA.org:oru-33947DiVA, id: diva2:699116
Available from: 2014-02-26 Created: 2014-02-26 Last updated: 2017-10-17Bibliographically approved
In thesis
1. Development of bioassays for risk assessment of PAHs and PFASs in the environment
Open this publication in new window or tab >>Development of bioassays for risk assessment of PAHs and PFASs in the environment
2013 (English)Doctoral thesis, comprehensive summary (Other academic)
Place, publisher, year, edition, pages
Örebro: Örebro universitet, 2013. p. 72
Series
Örebro Studies in Biology, ISSN 1650-8793 ; 8
National Category
Environmental Sciences Biological Sciences
Research subject
Biology
Identifiers
urn:nbn:se:oru:diva-30068 (URN)978-91-7668-965-3 (ISBN)
Public defence
2013-10-11, Hörsalen, Billbergska huset, Örebro universitet, Fakultetsgatan 1, Örebro, 10:00 (English)
Opponent
Supervisors
Available from: 2013-07-30 Created: 2013-07-30 Last updated: 2017-10-17Bibliographically approved

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Westman, OlaLarsson, MariaEngwall, Magnus

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