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Conditioned media from macrophages of M1, but not M2 phenotype, inhibit the proliferation of the colon cancer cell lines HT-29 and CACO-2
Department of Health Sciences, Karlstad University, Karlstad, Sweden.
Department of Health Sciences, Karlstad University, Karlstad, Sweden.
Örebro University, School of Medicine, Örebro University, Sweden.
Department of Health Sciences, Karlstad University, Karlstad, Sweden.
2014 (English)In: International Journal of Oncology, ISSN 1019-6439, Vol. 44, no 2, p. 385-392Article in journal (Refereed) Published
Abstract [en]

Solid tumors are infiltrated by stroma cells including macrophages and these cells can affect tumor growth, metastasis and angiogenesis. We have investigated the effects of conditioned media (CM) from different macrophages on the proliferation of the colon cancer cell lines HT-29 and CACO-2. CM from THP-1 macrophages and monocyte-derived human macrophages of the M1 phenotype, but not the M2 phenotype, inhibited proliferation of the tumor cells in a dose-dependent manner. Lipopolysaccaharide and interferon gamma was used for differentiation of macrophages towards the M1 phenotype and CM were generated both during differentiation (M1(DIFF)) and after differentiation (M1). M1 and M1(DIFF) CM as well as THP-1 macrophage CM resulted in cell cycle arrest in HT-29 cells with a decrease of cells in S phase and an increase in G(2)/M phase. Treatment of HT-29 cells with M1(DIFF), but not M1 or THP-1 macrophage CM, resulted in apoptosis of about 20% of the tumor cells and this was accompanied by lack of recovery of cell growth after removal of CM and subsequent culture in fresh media. A protein array was used to identify cytokines released from M1 and M2 macrophages. Among the cytokines released by M1 macrophages, tumor necrosis factor alpha and CXCL9 were tested by direct addition to HT-29 cells, but neither affected proliferation. Our results indicate that M1 macrophages inhibit colon cancer cell growth and have the potential of contributing to reducing tumor growth in vivo.

Place, publisher, year, edition, pages
Athens, Greece: Spandidos , 2014. Vol. 44, no 2, p. 385-392
Keywords [en]
M1 macrophages, THP-1, colon cancer cell line, HT-29, CACO-2, M2 macrophages
National Category
Medical and Health Sciences Cancer and Oncology
Research subject
Oncology
Identifiers
URN: urn:nbn:se:oru:diva-34857DOI: 10.3892/ijo.2013.2203ISI: 000332687400005PubMedID: 24296981Scopus ID: 2-s2.0-84894448580OAI: oai:DiVA.org:oru-34857DiVA, id: diva2:714417
Note

Funding Agencies:

County Council of Värmland

Örebro University

Available from: 2014-04-28 Created: 2014-04-25 Last updated: 2018-08-27Bibliographically approved

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Delbro, Dick

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