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Characterization of CTX-M-producing Escherichia coli by repetitive sequence-based PCR and real-time PCR-based replicon typing of CTX-M-15 plasmids
Örebro University, School of Health and Medical Sciences, Örebro University, Sweden. Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, Örebro, Sweden.
Örebro University, School of Medicine, Örebro University, Sweden. Örebro University Hospital. Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, Örebro, Sweden.ORCID iD: 0000-0001-5939-2932
Örebro University, School of Medicine, Örebro University, Sweden.
Örebro University Hospital. Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, Örebro, Sweden.
2014 (English)In: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), ISSN 0903-4641, E-ISSN 1600-0463, Vol. 122, no 11, p. 1136-1143Article in journal (Refereed) Published
Abstract [en]

The emergence of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae is a major global concern. CTX-M is the dominating ESBL type worldwide, and CTX-M-15 is the most widespread CTX-M type. The dissemination of CTX-M appears to be in part due to global spread of the Escherichia coli clone O25b-ST131. However, the gene-encoding CTX-M is mainly located on mobile genetic elements, such as plasmids, that also promote the horizontal dissemination of the CTX-M genes. In this study, 152 CTX-M-producing E. coli isolated in 1999-2008 in Örebro County, Sweden, were typed using a commercial repetitive sequence-based PCR (the DiversiLab system), and the prevalence of ST131 was investigated by pabB PCR. Real-time PCR-based plasmid replicon typing was performed on 82 CTX-M-15-producing E. coli isolates. In general, the CTX-M-producing E. coli population was genetically diverse; however, ST131 was highly prevalent (27%), and the dominating clone in our area. The blaCTX -M-15 gene was mainly located on IncF plasmids (69%), but a relatively high proportion of IncI1 plasmids (29%) were also detected among E. coli with diverse rep-PCR patterns, indicating that horizontal transmission of IncI1 plasmids carrying blaCTX -M-15 may have occurred between different E. coli strains.

Place, publisher, year, edition, pages
Malden, USA: John Wiley & Sons, 2014. Vol. 122, no 11, p. 1136-1143
Keywords [en]
Escherichia coli, ESBL, ST131, plasmid
National Category
Medical and Health Sciences Microbiology
Research subject
Medicine
Identifiers
URN: urn:nbn:se:oru:diva-35271DOI: 10.1111/apm.12270ISI: 000344383200011PubMedID: 24735173Scopus ID: 2-s2.0-84922019604OAI: oai:DiVA.org:oru-35271DiVA, id: diva2:722488
Note

Funding Agencies:

Research Committee of the County Council of Örebro 

Nyckelfonden at Örebro University Hospital

Available from: 2014-06-09 Created: 2014-06-09 Last updated: 2018-06-07Bibliographically approved

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Önnberg, AnnaSöderquist, BoPersson, Katarina

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Önnberg, AnnaSöderquist, BoPersson, KatarinaMölling, Paula
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School of Health and Medical Sciences, Örebro University, SwedenSchool of Medicine, Örebro University, SwedenÖrebro University Hospital
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Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS)
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