oru.sePublikationer
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
In vitro bioassays for detecting dioxin-like activity: Application potentials and limits of detection, a review
Rhein Westfal TH Aachen, Dept Ecosystem Anal, Inst Environm Res, Aachen, Germany.
Rhein Westfal TH Aachen, Dept Ecosystem Anal, Inst Environm Res, Aachen, Germany.
Fed Inst Hydrol BFG, Dept Biochem G3, Koblenz, Germany.
Fed Inst Hydrol BFG, Dept Biochem G3, Koblenz, Germany.
Show others and affiliations
2014 (English)In: Science of the Total Environment, ISSN 0048-9697, E-ISSN 1879-1026, Vol. 487, 37-48 p.Article in journal (Refereed) Published
Abstract [en]

Use of in vitro assays as screening tool to characterize contamination of a variety of environmental matrices has become an increasingly popular and powerful toolbox in the field of environmental toxicology.

While bioassays cannot entirely substitute analytical methods such as gas chromatography-mass spectrometry (GC-MS), the increasing improvement of cell lines and standardization of bioassay procedures enhance their utility as bioanalytical pre-screening tests prior to more targeted chemical analytical investigations. Dioxin-receptor-based assays provide a holistic characterization of exposure to dioxin-like compounds (DLCs) by integrating their overall toxic potential, including potentials of unknown DLCs not detectable via e.g. GC-MS. Hence, they provide important additional information with respect to environmental risk assessment of DLCs.

This review summarizes different in vitro bioassay applications for detection of DLCs and considers the comparability of bioassay and chemical analytically derived toxicity equivalents (TEQs) of different approaches and various matrices. These range from complex samples such as sediments through single reference to compound mixtures. A summary of bioassay derived detection limits (LODs) showed a number of current bioassays to be equally sensitive as chemical methodologies, but moreover revealed that most of the bioanalytical studies conducted to date did not report their LODs, which represents a limitation with regard to low potency samples.

(C) 2014 Elsevier B.V. All rights reserved.

Place, publisher, year, edition, pages
Amsterdam: Elsevier, 2014. Vol. 487, 37-48 p.
Keyword [en]
TEQ-approach, LOD, Dioxin, Effect directed analysis, Exposure characterization
National Category
Environmental Sciences
Research subject
Enviromental Science
Identifiers
URN: urn:nbn:se:oru:diva-35827DOI: 10.1016/j.scitotenv.2014.03.057ISI: 000337259100005OAI: oai:DiVA.org:oru-35827DiVA: diva2:740539
Available from: 2014-08-25 Created: 2014-07-30 Last updated: 2017-10-17Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Engwall, Magnusvan Bavel, Bert
By organisation
School of Science and Technology
In the same journal
Science of the Total Environment
Environmental Sciences

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 305 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf