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Isolation and characterization of a Lysinibacillus strain B1-CDA showing potential for bioremediation of arsenics from contaminated water
Systems Biology Research Center, School of Bioscience, University of Skövde, Skövde, Sweden; The Life Science Center, School of Science and Technology, Örebro University, Örebro, Sweden.
Systems Biology Research Center, School of Bioscience, University of Skövde, Skövde, Sweden.
Dr. D. Y. Patil Biotechnology and Bioinformatics Institute, Dr. D. Y. Patil Vidyapeeth, Tathawade, Pune, India.
Örebro University, School of Science and Technology.ORCID iD: 0000-0001-7957-0310
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2014 (English)In: Journal of Environmental Science and Health. Part A: Toxic/Hazardous Substances and Environmental Engineering, ISSN 1093-4529, E-ISSN 1532-4117, Vol. 49, no 12, 1349-1360 p.Article in journal (Refereed) Published
Abstract [en]

The main objective of this study was to identify and isolate arsenic resistant bacteria that can be used for removing arsenic from the contaminated environment. Here we report a soil borne bacterium, B1-CDA that can serve this purpose. B1-CDA was isolated from the soil of a cultivated land in Chuadanga district located in the southwest region of Bangladesh. The morphological, biochemical and 16S rRNA analysis suggested that the isolate belongs to Lysinibacillus sphaericus. The minimum inhibitory concentration (MIC) value of the isolate is 500mM (As) as arsenate. TOF-SIMS and ICP-MS analysis confirmed intracellular accumulation and removal of arsenics. Arsenic accumulation in cells amounted to 5.0mg g(-1) of the cells dry biomass and thus reduced the arsenic concentration in the contaminated liquid medium by as much as 50%. These results indicate that B1-CDA has the potential for remediation of arsenic from the contaminated water. We believe the benefits of implementing this bacterium to efficiently reduce arsenic exposure will not only help to remove one aspect of human arsenic poisoning but will also benefit livestock and native animal species. Therefore, the outcome of this research will be highly significant for people in the affected area and also for human populations in other countries that have credible health concerns as a consequence of arsenic-contaminated water.

Place, publisher, year, edition, pages
2014. Vol. 49, no 12, 1349-1360 p.
Keyword [en]
Pollution, toxic metals, arsenics, bioremediation, bacteria, bioaccumulation
National Category
Environmental Sciences
Research subject
Enviromental Science
Identifiers
URN: urn:nbn:se:oru:diva-36537DOI: 10.1080/10934529.2014.928247ISI: 000340370000002PubMedID: 25072766Scopus ID: 2-s2.0-84905275614OAI: oai:DiVA.org:oru-36537DiVA: diva2:747268
Funder
Sida - Swedish International Development Cooperation AgencySwedish Research Council Formas
Note

Funding Agency:

Nilsson-Ehle (The Royal Physiographic Society in Lund) foundation in Sweden

Available from: 2014-09-16 Created: 2014-09-15 Last updated: 2017-10-17Bibliographically approved
In thesis
1. Bioremediation of Toxic Metals for Protecting Human Health and the Ecosystem
Open this publication in new window or tab >>Bioremediation of Toxic Metals for Protecting Human Health and the Ecosystem
2016 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Heavy metal pollutants, discharged into the ecosystem as waste by anthropogenic activities, contaminate drinking water for millions of people and animals in many regions of the world. Long term exposure to these metals, leads to several lethal diseases like cancer, keratosis, gangrene, diabetes, cardio- vascular disorders, etc. Therefore, removal of these pollutants from soil, water and environment is of great importance for human welfare. One of the possible eco-friendly solutions to this problem is the use of microorganisms that can accumulate the heavy metals from the contaminated sources, hence reducing the pollutant contents to a safe level.

In this thesis an arsenic resistant bacterium Lysinibacillus sphaericus B1-CDA, a chromium resistant bacterium Enterobacter cloacae B2-DHA and a nickel resistant bacterium Lysinibacillus sp. BA2 were isolated and studied. The minimum inhibitory concentration values of these isolates are 500 mM sodium arsenate, 5.5 mM potassium chromate and 9 mM nickel chloride, respectively. The time of flight-secondary ion mass spectrometry and inductively coupled plasma-mass spectroscopy analyses revealed that after 120 h of exposure, the intracellular accumulation of arsenic in B1-CDA and chromium in B2-DHA were 5.0 mg/g dwt and 320 μg/g dwt of cell biomass, respectively. However, the arsenic and chromium contents in the liquid medium were reduced to 50% and 81%, respectively. The adsorption values of BA2 when exposed to nickel for 6 h were 238.04 mg of Ni(II) per gram of dead biomass indicating BA2 can reduce nickel content in the solution to 53.89%. Scanning electron micrograph depicted the effect of these metals on cellular morphology of the isolates. The genetic composition of B1-CDA and B2-DHA were studied in detail by sequencing of whole genomes. All genes of B1-CDA and B2-DHA predicted to be associated with resistance to heavy metals were annotated.

The findings in this study accentuate the significance of these bacteria in removing toxic metals from the contaminated sources. The genetic mechanisms of these isolates in absorbing and thus removing toxic metals could be used as vehicles to cope with metal toxicity of the contaminated effluents discharged to the nature by industries and other human activities.

Place, publisher, year, edition, pages
Örebro: Örebro university, 2016. 80 p.
Series
Örebro Studies in Life Science, 15
Keyword
Heavy Metals, Pollution, Accumulation, Remediation, Human Health, Bacteria, Genome Sequencing, de novo Assembly, Gene Prediction
National Category
Other Biological Topics
Research subject
Biology
Identifiers
urn:nbn:se:oru:diva-51436 (URN)978-91-7529-146-8 (ISBN)
Public defence
2016-09-22, Högskolan i Skövde, G-building, lecture hall G111, Högskolevägen 28, Skövde, 13:15 (English)
Opponent
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Available from: 2016-07-25 Created: 2016-07-25 Last updated: 2017-10-17Bibliographically approved

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