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Determination of chitin in fungi and mycorrhizal roots by an improved HPLC analysis of glucosamine
University of Umeå, Umeå, Sweden.ORCID iD: 0000-0003-4384-5014
Swedish University of Agricultural Sciences, Umeå, Sweden.
1996 (English)In: Plant and Soil, ISSN 0032-079X, E-ISSN 1573-5036, Vol. 178, no 1, 29-35 p.Article in journal (Refereed) Published
Abstract [en]

A method to measure chitin content in fungi and ectomycorrhizal roots with high-performance liquid chromatography (HPLC) was developed. Measurements of fluorescence of 9-fluorenylmethylchloroformate (FMOC-CI) derivatives of glucosamine were made on acid hydrolysates of pure chitin, chitin-root mixtures and fungal-root mixtures. The method was applied on 5 isolates of ectomycorrhizal fungi, and ectomycorrhizal and non-mycorrhizal Pinus sylvestris roots. Interference from amino acids was removed by pre-treatment of samples with 0.2 N NaOH. This pre-treatment did not reduce the recovery of chitin, nor did plant material affect the recovery of chitin. The HPLC method was compared with a colorimetric chitin-method by measurements on root-fungal mixtures, with known fungal content. The HPLC method gave estimates of fungal biomass which were equal to the expected while the colorimetric method showed values significantly (p<0.001) lower than the expected. The present chitin method offers a sensitive and specific tool for the quantification of chitin in fungi and in ectomycorrhizal roots.

Place, publisher, year, edition, pages
1996. Vol. 178, no 1, 29-35 p.
Keyword [en]
chitin, ectomycorrhizal fungi, glucosamine, high-performance liquid chromatography
National Category
Botany
Identifiers
URN: urn:nbn:se:oru:diva-37412DOI: 10.1007/BF00011160ISI: A1996TX35100002OAI: oai:DiVA.org:oru-37412DiVA: diva2:751944
Available from: 2014-10-02 Created: 2014-10-02 Last updated: 2017-10-17Bibliographically approved

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