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X-Ray Microanalysis in the Scanning Electron Microscope
Örebro University, School of Medicine, Örebro University, Sweden.
Department of Pathology, Uppsala Academic Hospital, Uppsala, Sweden .
2014 (English)In: Electron microscopy: methods and protocols / [ed] John Kuo, New York, USA: Humana Press, 2014, 3, 639-661 p.Chapter in book (Refereed)
Abstract [en]

X-ray microanalysis conducted using the scanning electron microscope is a technique that allows the determination of chemical elements in bulk or semi-thick specimens. The lowest concentration of an element that can be detected is in the order of a few mmol/kg or a few hundred parts per million, and the smallest amount is in the order of 10(-18) g. The spatial resolution of the analysis depends on the thickness of the specimen. For biological specimen analysis, care must be taken to prevent displacement/loss of the element of interest (usually ions). Protocols are presented for the processing of frozen-hydrated and freeze-dried specimens, as well as for the analysis of small volumes of fluid, cell cultures, and other specimens. Aspects of qualitative and quantitative analysis are covered, including limitations of the technique.

Place, publisher, year, edition, pages
New York, USA: Humana Press, 2014, 3. 639-661 p.
Series
Methods in molecular biology, ISSN 1064-3745 ; 1117
Keyword [en]
X-ray microanalysis, Scanning electron microscopy, Peak-to-background ratio, Frozen-hydrated specimen, Sephadex beads, Cell culture, Airway surface liquid
National Category
Biochemistry and Molecular Biology
Research subject
Biochemistry; Molecular Biology
Identifiers
URN: urn:nbn:se:oru:diva-37564DOI: 10.1007/978-1-62703-776-1_28PubMedID: 24357383Scopus ID: 2-s2.0-84934434731ISBN: 978-1-62703-776-1 (print)ISBN: 978-1-62703-775-4 (print)OAI: oai:DiVA.org:oru-37564DiVA: diva2:753108
Available from: 2014-10-07 Created: 2014-10-07 Last updated: 2017-10-17Bibliographically approved

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Roomans, Godfried M.
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CiteExportLink to record
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Citation style
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